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EN
Problems in polytherapy of hypertension combined with hyperlipidemia and the need of application of several pharmaceutical preparations have been widely explored by patients, physicians, and the pharmaceutical industry through testing and introduction of a fixed-dose-combination products (FDC). The paper presents different analytical methods of qualitative and quantitative analysis or stability testing of FDC preparations and laboratory prepared mixtures of composition consistent with the FDC products collected on the basis of publications from the last decade. It can significantly facilitate the study of the methods and conditions for the determination of active substances in polypills which contain at least three APIs, especially in cases involving this type of preparations used or tested for their application in polytherapy of hypertension.
EN
The purpose of this study was to determine the difference in five kinematic variables (internal knee and elbow angles, elbow height, forearm angle from vertical, and shoulder flexion angle at ball release) between proficient and non-proficient free throw shooters and which variables had the greatest contributions to a successful free throw shooting outcome. Seventeen male basketball players shoot three sets of 10 free throws with a two-minute break between each set. A three-dimensional motion tracking system composed of 17 sensors sampling at 60 Hz was used for data collection. Proficient free throw shooters had greater knee and elbow flexion, lower elbow height, and a smaller forearm angle compared to non-proficient shooters. These results explained 89.5% of the total variance. While maintaining the optimal range of these kinematic variables allows each subject to reach an appropriate level of free throw shooting performance, the key variable capable of distinguishing between made and missed shots within the proficient group of shooters was the forearm angle. Positioning the forearm parallel, or close to parallel, with an imaginary vertical line during the preparatory phase of the shooting motion accounted for 23.9% of the total variance and was associated with a greater number of made shots.
EN
In recent decades, a large number of targeting drug delivery systems have been designed and evaluated for cancer therapy. An important part of these studies is to monitor the subcellular fate of macromolecules. Fluorescence labeling technique was the most adopted method. However, it had the disadvantages of alteration the properties of drugs as well as artifactual redistribution of fluorescent markers caused by fixation. In the present study, a more accurate method, HPLC analysis preceded by subcellular fractionation was developed and validated to study the distribution of a newly synthesized tumor cell targeting drug delivery system, HPMA copolymer-5-FU conjugates (P-FU) in PC-3 cells. P-FU was proved to accumulate more in nuclei than free 5-FU, whose concentration in the culture medium was approximately 1/20 that of 5-FU when attaining similar nucleus drug concentration. On the contrary, the overwhelming majority of drugs accumulated in nonnucleus when PC-3 cells were treated with free 5-FU. These results indicated that P-FU had nuclear targeting effect. The developed procedure allows an accurate subcellular fate screening for many macromolecules.
EN
This paper presents an empirical approach to the changes in motor skills of children who train sports swimming at the initial stage of school education in an annual training cycle. Swimming belongs to the disciplines in which training starts at the age of 6-7. The proper selection of candidates to train certain disciplines is a complex process as they should be chosen from a large population of children, both girls and boys, having specific somatic and motor characteristics which, developed in the long-term, will enable them to achieve sports mastery. The aim of the research was to define which changes in motor skills occur in girls who train sports Aim: swimming in an annual training cycle. The Subject group consisted of 85 girls aged 7 who attended four elementary schools in Szczecin, Poland. 36 of them belonged to the Swimmers group and they were all members of the Municipal Swimming Club (MKP) in Szczecin. The Control group consisted of 49 girls who attended the same elementary schools. All subjects took part in two examinations (carried out in the school year 2009/2010. Physical ability tests were conducted in gyms. Motor skills were assessed with EUROFIT Test Battery which is the most reliable and accurate tool according to scientific research. The research revealed changes in both groups (Sw, C) in terms of all eight tests. Examination II proved statistically significant improvement of results in both groups (Sw, C) in comparison to Examination I. Changes between Examination I and Examination II results were most visible in the Swimmers groups in terms of balance, agility, static strength, functional strength and agility run. Changes between Examination I and Examination II were similar in both groups (Sw, C) in terms of speed of limb movement, explosive strength and torso strength. Progressive changes in motor skill of subjects are a positive phenomenon in the physical development of a child. Swimming training resulted significantly in positive changes in terms of motor skills of subject who were at the initial stage of swimming trainings, compared to their non-training peers. Participation in organized, regular sports classes results in the development of motor (physical) skills of children.
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EN
There were presented technological and ecological aspects of biogas quality evaluation and introduced an overview of the limits of its selected parameters. There were characterized analytical methods for determining the basic composition of biogas, hydrogen sulfide, ammonia, condensation, dust, oils, siloxanes, and aggregated content of sulfur, chlorine and fluorine. The result of comparative analysis and literature studies are proposes of the most adequate to the studies of biogas - analytical methodologies.
PL
Przedstawiono technologiczne i ekologiczne aspekty oceny jakości biogazu oraz zaprezentowano przegląd granicznych wartości jego wybranych parametrów. Scharakteryzowano metody analityczne oznaczania składu podstawowego biogazu, siarkowodoru, amoniaku, kondensatu, pyłów, olejów, siloksanów oraz sumarycznych zawartości związków siarki, chloru i fluoru. Efektem przeprowadzonych analiz porównawczych i badań literaturowych są propozycje metodyk analitycznych najbardziej adekwatnych do badań biogazu.
EN
Introduction: Swimming is one of the most popular sports practiced by people with disabilities, and the number of participants during the subsequent Paralympic Games (PG) increases. One way to assess the level of sports in a given discipline is to analyze the sports results of the athletes competing at the highest level. Hence, the purpose of analysis was to compare the results obtained by the athletes with locomotor disability in swimming in the starting class (1-10) on the PG in the years 2000-2012. Material and methods: Were analyzed the results obtained by men with locomotor disability in swimming all styles, on all distances during the PG in 2000-2012. To assess the significance of differences between the results obtained by the swimmers in the analyzed period the Mann-Whitney U test were used. The level of significance was set at p <0.05. Results: We observed statistically significant differences in the level (increase) of obtained results in all classes and styles, on distances played during the next PG. Throughout the 8 and 12 years, almost all differences were statistically significant. This direction of change was most evident in case of athletes from higher start classes (6-10). Conclusions: The results indicate a steady increase in the sports results of men with locomotor disability in swimming. It could be caused by much interest in this form of activity among people with disabilities, especially those with less functional deficit. Attention should be paid to increasing the share of the athletes from low-start classes (1-5).
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