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1

Transgenic lines of rape resistant to infection of turnip mosaic virus (tum

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Lehmann P., Kozubek E., Wojciechowski A.
Biotechnologia
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1998
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issue 1
129-139
EN
Transgenic Brassica napus var. oleifera cv. Westar plants that express the coat protein gene of the turnip mosaic virus (TuMV) were generated. The transgenic plant lines that contained one, two and at least three transgenes inserts in both homozygous and hemizygous conditions were produced and examined. Twelve different transgenic plant lines (in T3 generation) were analysed for resistance to TuMV. Three different responses were observed when the transgenic plant lines were inoculated with TuMV; 1) some were highly reistant, 2) some were medium resistant and, 3) some were susceptible to TuMV infection. We did not find any correlation between the number of copies of the transgene and virus resistance. The most promissing transgenic line was used to breed a few winter cultivars of rape to induce the pathogen-mediated virus resistance in these cultivars.
2

Susceptibility of Polish winter oilseed rapes to turnip mosaic virus. I. Virus detection in leaves of infected plants by ELISA test

100%
Ostrowka K., Lehmann P., Walsh J. A., Kozubek E.
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vol. 34
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issue 2
153-157
EN
Eight Polish forms: four double haploid lines, two strains and two cultivars were inoculated with (TuMV). All plants appeared to be susceptible and expressed systemic reaction: mosaic and malformation of leaves. All forms but two showed considerable growth retardation. The level of TuMV infection of plants was estimated by indirect . High concentration of the virus was found in all inoculated tested plants.
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