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1

Fluorescence-based AFLPs occur as the most suitable marker system for oilseed rape cultivar identification

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Sobotka R., Dolanska L., Curn V., Ovesna J.
Journal of Applied Genetics
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2004
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vol. 45
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issue 2
161-173
EN
Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showed that the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape cultivars, 60 RAPD primers were tested and only eight of them (14%) detected sufficient levels of polymorphism. Five microsatellites out of fifteen tested were polymorphic, but in all loci, except one, only two different alleles were detected. This result indicated the limited degree of polymorphism found in Brassica napus. Each of the six tested AFLP combinations detected polymorphisms, the best combination (M-CAA/E-ACT) had 26% polymorphic peaks from a total of 90 peaks and could distinguish the analysed cultivars and 4 out of 5 core lines of cultivars. The results presented show that florescence-based AFLP is, for the purposes of oilseed rape cultivar fingerprinting, a more suitable approach than either RAPD or SSR.
2

Transgenic lines of rape resistant to infection of turnip mosaic virus (tum

100%
Lehmann P., Kozubek E., Wojciechowski A.
Biotechnologia
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1998
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issue 1
129-139
EN
Transgenic Brassica napus var. oleifera cv. Westar plants that express the coat protein gene of the turnip mosaic virus (TuMV) were generated. The transgenic plant lines that contained one, two and at least three transgenes inserts in both homozygous and hemizygous conditions were produced and examined. Twelve different transgenic plant lines (in T3 generation) were analysed for resistance to TuMV. Three different responses were observed when the transgenic plant lines were inoculated with TuMV; 1) some were highly reistant, 2) some were medium resistant and, 3) some were susceptible to TuMV infection. We did not find any correlation between the number of copies of the transgene and virus resistance. The most promissing transgenic line was used to breed a few winter cultivars of rape to induce the pathogen-mediated virus resistance in these cultivars.
3

Some morphological and phenological traits and fertility of lines of artificial winter oilseed rape originated from male-sterile plants (Barssica napus var.olifera L.)

100%
Wojciechowski A.
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vol. 34
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issue 4
317-325
4

Analyses of air samples for ascospores of Leptosphaeria maculans and L.biglobosa by light microscopy and molecular techniques

100%
Kaczmarek J., Jedryczka M., Fitt B. D. L., Lucas J. A., Latunde-Dada A. O.
Journal of Applied Genetics
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2009
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vol. 50
|
issue 4
411-419
EN
Spores of many fungal pathogens are dispersed by wind. Detection of these airborne inocula is important in forecasting both the onset and the risk of epiphytotics. Species-specific primers targeted at the internal transcribed spacer (ITS) region of Leptosphaeria maculans and L. biglobosa ? the causal organisms of phoma stem canker and stem lesions of Brassica spp., including oilseed rape ? were used to detect DNA extracted from particles deposited on tapes obtained from a spore trap operated in Rarwino (northwest Poland) from September to November in 2004 and 2006. The quantities of DNA assessed by traditional end-point PCR and quantitative real-time PCR were compared to microscopic counts of airborne ascospores. Results of this study showed that fluctuations in timing of ascospore release corresponded to the dynamics of combined concentrations of DNA from L. maculans and L. biglobosa, with significant positive correlations between ascospore number and DNA yield. Thus the utilization of PCR-based molecular diagnostic techniques enabled the detection, identification, and accurate quantification of airborne inoculum at the species level. Moreover, real-time PCR was more sensitive than traditional PCR, especially in years with low ascospore numbers.
5

Susceptibility of Polish winter oilseed rapes to turnip mosaic virus. I. Virus detection in leaves of infected plants by ELISA test

100%
Ostrowka K., Lehmann P., Walsh J. A., Kozubek E.
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vol. 34
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issue 2
153-157
EN
Eight Polish forms: four double haploid lines, two strains and two cultivars were inoculated with (TuMV). All plants appeared to be susceptible and expressed systemic reaction: mosaic and malformation of leaves. All forms but two showed considerable growth retardation. The level of TuMV infection of plants was estimated by indirect . High concentration of the virus was found in all inoculated tested plants.
6

The practical use of molecular markers in hybrid breeding of winter oilseed rape (Brassica napus L. var. oleifera)

100%
Poplawska W., Liersch A., Bartkowiak-Broda I.
Biotechnologia
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2004
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issue 2
17-22
EN
Breeding of oilseed rape hybrid varieties in Poland is based on CMS ogura hybrydization system. The marker assisted selection is used in selection of parental lines of F1 hybrids. The markers of alleles of restorer gene Rfo are the most important in breeding programs. Also, the investigations on genetic distance of hybrid parental lines using molecular markers are undertaken aiming at its application for preliminary selection of F1 combinations.
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