Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 10

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

Search:
in the keywords:  IMMOBILIZATION
help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Production of ethanol from lactose by yeast cells immobilized in open-pore agar

100%
Szczodrak J., Szczodrak Z., Wiater A.
Biotechnologia
|
1997
|
issue 4
82-93
EN
An open-pore agar matrix has been shown to be suitable for the entrapment of Candida pseudotropicalis whole cells are used in reactions that involve cell growth and gas evolution. The basic conditions for lactose fermentation by immobilized yeast cells i.e. inoculum quantity, pH, temperature and culture time were standardized. It was shown that yeast cells immobilized in the porous carrier could be used repeatedly in the batch fermentation system for seven 36 h cycles without any substantial loss in ethanol yield, and beads of porous agar with entrapped cells did not rupture, even after periods of 18 d of use.
2

Membrane reactor - new possibilities

80%
Noworyta A., Bryjak J.
Biotechnologia
|
1993
|
issue 3
4-15
EN
The flow-sheet of for biotechnological application has been presented in the paper. The relevant topics that concern problems of microbial or as well as separation of reaction system onto the fractions with different residence time distributions have been overiewed. A set of conditions has been specified for membranes applied in the system. The following types of bioreactors have been characterized in detail i.e.:i) case of bioreactor for hydrolysis of penicillinum G and ii) case of microbial membrane bioreactor for culture and harvesting of biomass iii) microbial membrane bioreactor for alcohol fermentation.
3

The application of immobilized invertase for continuous production of inverted sucrose

80%
Ginalska G., Belcarz A., Lobarzewski J., Wolski T.
Biotechnologia
|
1999
|
issue 1
226-237
EN
Four inorganic supports (sand, silica gel, crushed pumice and controlled-pore glass) were used for immobilization of invertase. The possibility of application of thus obtained immobilized enzyme in continuous production of inverted sugar was tested. The best support for immobilization of invertase was controlled-pore glass (CPG, average pore size of 31 mm). The conditions of production of inverted sugar by immobilized enzyme were optimised (also in column). Unter the optimised the column (dimensions: 0.7x27 cm) with immobilized invertase was able to degrade sucrose to glucose and fructose in an amount of 60 g/h. Immobilized invertase in the column remained fully active for 120 days.
4

Enzymatic production of maltodextrins and syrup solids from starch. Part II. Processes

80%
Bryjak J.
Biotechnologia
|
1999
|
issue 1
201-225
EN
This part mainly discusses the enzymatic processes employed in the production of maltodextrins and syrup solids. Functional properties and applications of dextrins, maltose, glucose, fructose, cyclodextrins as well as malto- and isomaltooligosaccharides are presented. Immobilization of amylolytic enzymes and typical enzyme reactors for starch processing is reviewed. Action pattern of amylases in relation to the subsite structure of the enzyme active domains is presented, including: the dependence of Michaelis and molecular activity parameters on the DP of substrate; possible binding modes between the enzyme and a linear substrate; multiple attack mode in addition to simple hydrolysis (random attack). Mathematical modelling of starch degrading processes is critically reviewed. The crucial parameters, influencing the reaction rate and the conversion degree of the substrate, are shown.
5

Kinetics of biological iron oxidation in immobilized packed-bed reactors

80%
Nawrocki J., Wisniewski P., Grajek W., Astrib M.
Biotechnologia
|
1995
|
issue 2
141-152
EN
A packed-bed bioreactors with activated carbon particles as a cerrier for iron-oxidizing bacteria was used for ferrous sulfate oxidation.The bioreactors were operated continuously for about 2 weeks.The experimental variables were: dillution rate, pH, medium composition and Fe(II) ions concentration.It was found that the efficiency of iron oxidation depended on the dilution rate and the medium pH.As dilution rate increased, the oxidation rate was decreased but at the same time reacto productivity was improved.The highest kinetic performance achieved in single stage fermentation related to the Fe(II) oxidation was over 90% when D was below 0.2/h.The pH optimum was 1.3. At higher pH values a ferric iron precipitation was accelerated.It was showed thet replacement of the 8K medium with tap water has not had a negative effect on iron oxidation process.
6

Characteristics of the ability to uptake cadmium by free and immobilized bacteria Kliebsiella pneumoniae

70%
Wojnowska-Baryla I., Stolarczyk E., Gredzinska A.
Biotechnologia
|
1997
|
issue 4
103-112
EN
The ability to uptake cadmium of the following biosorbents: alginate gel, free and immobilized in alginate bacteria Kliebsiella pneumoniae was characterized. An average efficiency of cadmium removal from solution with initial cadmium concentration range from 1 to 200 mg Cd2+?dm-3 was 78,6% by alginate gel and immobilized bacteria Kliebsiella pneumoniae and 60% by free bacteria. The maximum efficiency of cadmium removal from solution with initial concentration 1 mg Cd2+? dm-3 was obtained for bacteria immobilized in alginate gel.
7

Batch propionic acid fermentation using propionibacterium sp. immobilized in different supports

70%
Czaczyk K., Trojanowska K.
Biotechnologia
|
1998
|
issue 1
188-198
EN
Propionibacterium freudenreichii subsp. shermanii 1 and 4 were immobilized in the living state in 2, 3 and 4% alginate gels, in 2, 4 and 6% carrageenan gels, in 2, 4 and 6% carrageenan/locust bean gum gels and on ceramic support. Ammonia consumption, glucose utilization, production of propionic and acetic acids, biosynthesis of vitamin B12 and cell release rate were examined. A significant increase of productivity of propionic acid and decrease of biosynthesis of vitamin B12 using immobilized cells were observed. The best results were obtained in the fermentation with strains immobilized in 4% alginate gel, when applied for the third time. In this case, production of propionic acid was 50% higher in comparison with free cells and biosynthesis of vitamin B12 was lower or the same as in the control.
8

Age-dependent effect of limb immobilization and remobilization on rat bone

70%
Trebacz H., Dmowska M., Baj J.
Folia Biologica
|
2002
|
vol. 50
|
issue 3-4
121-127
EN
The effect of unilateral hindlimb immobilization and subsequent free remobilization on bone tissue in rats was examined. Right hindlimb of intensively growing (G), young adult (Y) and adult (A) male rats was immobilized by taping for two weeks. Bone tissue was investigated post mortem in experimental and age-matched control rats, either directly after immobilization (Imm) or after two or four weeks of remobilization (Re2, Re4). Apparent density (dapp) and mineralization (Min) were estimated in femora and pelvis. The mechanical state of bone tissue in femora was evaluated using an ultrasonic method. Additionally, activity of serum alkaline phosphatase, and serum calcium and phosphorus were measured in each group. Min and dapp in Imm bones were changed in G rats, while in Y and A only dapp in Imm femora was affected. Velocity of ultrasound was significantly lower in immobilized femora in each age group, indicating decreased elasticity of bone tissue. The differences between immobilized and control limbs were still significant in Re2 and Re4 groups in G rats. In Y rats the differences between experimental and control bones increased during remobilization. It is concluded that deterioration of bone initiated during two weeks of unloading, last within at least four weeks of free remobilization, despite restoration of normal activity.
9

Encapsulation of immobilised in situ intracellurar lipases of Mucor

61%
Antczak T., Bugla J., Szeja W., Galas E.
Biotechnologia
|
1999
|
issue 1
172-179
EN
Endolipases associated with the cell structures of microorganisms are more active in comparison with purified enzymes. Due to the weak mechanical resistance lipases immobilised in situ cannot be used many times. The method of lipases encapsulation in polysacharides hydrophilic gels was elaborated. The lipases Mucor immobilised in situ were treated with oleic acid in hexane and then dispersed in an aqueous solution of sodium alginate or karagenate. Immobilisation of enzymes was achieved by intermolecular cross-linking of the polysacharide chains using the solution of calcium or potassium salts. The biocatalysts prepared under proposed conditions were active in hydrolysis of esters, as well as in esterification reaction. It was found that immobilised enzymes were active for a long time, were mechanically resistant and could be used many times in periodic and continuous processes.
10

Entrapment of immobilised in situ intracellular lipase of Mucor in microporous capsules

61%
Antczak T., Bugla J., Mastalerz M., Niemiec A., Szeja W., Slek M., Galas E.
Biotechnologia
|
2000
|
issue 4
142-151
EN
Lipases Mucor circinelloides and Mucor racemosus immobilised in situ were closed in microporous polysacharides hydrophilic gel cross-linked using a solution of calcium salts. In order to increase the porosity of polysacharides matrix during its cross-linking oligomer molecules of ethylene oxide (optimal Polikol 1000) were incorporated in the structure of the matrix. Then the oligomer was removed by acetone extraction. The obtained biocatalyst preparations were tested in hydrolysis of esters and esterification of oleic acid with butanol. The hydrolysis was carried out in water saturated organic solvents medium (n-hexane and diisopropylether). It was found that the efficiency of M. racemosus lipase immobilisation in hydrolysis of n-butyl oleate, n-butyl palmitate, n-butyl stearate, n-butyl laurate amounted to 60%. The efficiency of M. circinelloides lipase immobilisation in esterification of oleic acid with 1-butanol in hexane achieved 45%.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.