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1
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Dysmutaza ponadtlenkowa, katalaza i peroksydaza glutationowa w trisomicznych fibroblastach ludzkich z hodowli in vitro

100%
Rózga B., Uniwersytet Łódzki K. T.
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
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1998
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vol. 13
EN
The activities of intercellular antioxidative enzymes: Superoxide dismutase, catalase and glutathione peroxidase were estimated in control and trisomic human fibroblasts in culture. It was found that in trisomic cells the activity of SOD is approximately 50% higher than in control cells. The activity of glutathione peroxidase is increased by 30% in trisomic fibroblasts, whereas catalase activity is similar in both types of cells.
2
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Pojedyncze pęknięcia DNA fibroblastów od osób z zespołem Downa

100%
Rózga B., Uniwersytet Łódzki K. B.
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
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1992
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vol. 9
3
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Cytometryczna metoda oznaczania żywotności komórek limfoidalnych w warunkach stresu temperaturowego

51%
Zaborowski A., Klink M., Rózga B., Andrzej Zaborowski - Uniwersytet Łódzki K. B. M., Magdalena Klink - Uniwersytet Łódzki K. T., PAN B. R. C. M. i. W.
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
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1994
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vol. 10
EN
The viability of freshly isolated or heat-treated lymphocytes was estimated by the classical microscopic method based on trypan blue inclusion and it was measured by flow cytometry of the cells stained with acridine orange and ethydium bromide or with acridine orange alone. It has been showed that (low cytometry of fluorescence stained cells allowed to determine the viability of the cell suspensions containing even a small percentage of dead cells rapidly and accurately.
4
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Degradation of 3H-TdR labelled DNA of human lymphocytes during storage

51%
Rózga B., Poziomska E., Koceva-Chyła A., University of Łódź I. o. B. a. B.
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
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1983
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vol. 2
5
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Zastosowanie dwuchlorowodorku kwasu 3,5-dwuaminobenzoesowego do fluorymetrycznej identyfikacji DNA limfocytów ludzkich w alkalicznym gradiencie gęstości sacharozy

45%
Rózga B., Koceva-Chyła A., Poziomska E., Tranda T., Leyko W., Uniwersytet Łódzki I. B. i. B.
Acta Universitatis Lodziensis. Folia Biochimica et Biophysica
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1981
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vol. 1
EN
The fluorimetrio method with DABA x 2HC1 was applied in the work in localizlng the DNA in fractions of alkaline sucrose density gradient. The human lymphocytes used in the experiments were exclted towards the blastic transformation wlth phitohemaglutinine (PHA+), the unexoitated human lymphocytes (PHA-) and the commercise samcle of DNA izola ted frora the calf thymus (Sigma Chem. Co) were also usod. The obtalned data show that the results of the fluorimetric assay of the human lymphocytes DNA using the DABA x 2HCl in the fractions of the sucrose density gradient are in agreement with results obtained using the radioizotope method,' both for the unirradiated as well as for irradiated lymphocytes, and that this method might be used in the estimations of the molecular weight of the DNA using the centrifugation method in the sucrose density gradients without previous labelling of the cells.
DE
In der vorliegenen Arbeit verwendete man die fluorymetrischen Methoden aus DABA x 2HCl zur Bezeichnung der DNS-Lokalisierung in den Fraktionen des alkalischen Gradients der Dichtigkeit der Sacharose. Man gebrauchte die menschlichen Lymphocyten, die zur blastlschen Transformation mit Hilfe von Fitohemaglutyninum (PHA+) angeregt wurden, diś nicht angeregten menschlichen Lymphozyten (PHA-) und das geziichtete Praparat der DNS des Kalbsthymus. (Sigma Chem. Co). Die gewonnen Angaben zeigten, dass die Ergebnisse der fluorymetrischen Kennzeichnung DNS der menschlichen Lymphocyten bei der Verwendung von DABA x 2HC1 in den Fraktionendes alkalischen Gradients der Dichtigkeit von Sacharose mit den Ergebnissen, die mit Hilfe der radioizotopischen Methode iibereinstimmen, sowohl im Fali der bestrahlten Lymphozyten ais auch der unbestrahlen. Diese Methode kann bei den Untersuchungen der Masse von Molekiilen der DNS mit Hilfe des Wirbelins in den Gradienten der Dichtigkeit der Sacharose verwendet werden,, ohne die Notwendichkeit der friiheren Kennzeichnung der Zellen.
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