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Hemoglobiny bakterii

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The aim of this paper is to summarize the publications concerning the bacterial hemoglobin (Vitreoscilla). This is the first bacterial hemoglobin to be sequenced and physicochemical properties are determined. It showed the maximum sequence homology (24%) with lupin leghemoglobin (Lg) which properties also have been presented. Bacterial hemoglobin may function to enable the organism to survive in oxygen-limited environments by acting as an oxygen storage-trap or to facilitate oxygen diffusion.
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The paper contains editorial and stylistic requirements posed to diploma dissertations in the range of molecular biology. The instructions and remarks given should help students preparation of the first scientific account i.e. their Magister dissertation.
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In invertebrates various proteins play the role of oxygen transporter. They are dissolved in fluids of the respiratory system or present in its cells. These proteins occur also in other tissues, and therefore are called tissue hemoglobins. They play an important role evidenced by occurrence in tissues of animals possessing no other respiratory proteins in their blood or hemolymph. This paper presents the occurrence of hemoglobins in the animal kingdom and their physico-chemical properties, with special attention paid to the oxygen affinity.
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Oxygen affinity of respiratory proteins is characterized by three parameters: P50, n, K (terms of the Hill plot). They are derived from oxygen dissociation curves determined usually by the spectrophotometric method. The allosteric properties of respiratory proteins are examined by comparing the experimental data to MWC model. A computer program was written and the program calculated the rood mean square deviation between the data points (Amstrad CPC 464). From the lower and upper asymptotes of the Hill plots the 1CK and K1 values could be obtained as a first approximation. The computer-fitting program allowed to determine the allosteric parameters.
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This paper presents an outline of the activity of Laboratory of Biological Sciences of the University of Łódź during the first ten years of its existence (1974-1984).
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