One great advantage of liposome-encapsulated genes for gene theraphy is its safeness.But a shortcoming is its low transfection effeciency.To overcome this shortage, we devised cationic large uniamellar vesicles for efficient and transfer of the gene for its expression.Recently, we devised a simple method to prepare cationic multilamellar large vesicles.By use of these vesicles, malignant glioma in an experimental model was successfully cured.