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Journal
1997 | 4 | 71-81
Article title

Influence of DNA concentration on the efficiency of bovine blastocyst production after WAP-bGH microinjection into germinal vesicle of immature oocytes, zygotes and embryos obtained in vivo or in vitro

Title variants
Languages of publication
EN
Abstracts
EN
Microinjection is one of the most successfully used methods to produce transgenic farm animals. But the effectiveness of transgenesis with the use of microinjection, specially in cattle is still low. Many steps of the transgenesis has been found to influence its effectiveness; DNA purity, the site of its injection, the culture system. There are no reports on the influence of a DNA vector concentration influence on the developmental ability of transformed bovine eggs to the blastocyst stage in the in vitro culture. In presented experiments we investigated the influence of different DNA concentrations on the developmental rate of microinjected immature bovine oocytes, zygotes and 2-cell embryos to the blastocyst stage.
Publisher

Journal
Year
Issue
4
Pages
71-81
Physical description
Contributors
author
author
author
author
References
Document Type
article
Publication order reference
J. Jura, National Research Institute of Animal Production, Department of Animal Reproduction, 32-083 Balice/Krakow, Poland, fax (012) 856733
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-f565a3f3-0737-3794-9e42-0cb8f7e1bd5b
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