Influence of DNA concentration on the efficiency of bovine blastocyst production after WAP-bGH microinjection into germinal vesicle of immature oocytes, zygotes and embryos obtained in vivo or in vitro

• Authors: J. Jura , Z. Smorag , L. Katska , M. Skrzyszowska , L-M. Houdebine , B. Rynska
• Languages of publication: EN

Abstracts

EN

Microinjection is one of the most successfully used methods to produce transgenic farm animals. But the effectiveness of transgenesis with the use of microinjection, specially in cattle is still low. Many steps of the transgenesis has been found to influence its effectiveness; DNA purity, the site of its injection, the culture system. There are no reports on the influence of a DNA vector concentration influence on the developmental ability of transformed bovine eggs to the blastocyst stage in the in vitro culture. In presented experiments we investigated the influence of different DNA concentrations on the developmental rate of microinjected immature bovine oocytes, zygotes and 2-cell embryos to the blastocyst stage.

Keywords

EN

BOVINE IMMATURE OOCYTE; CELL EMBRYO; DNA CONCENTRATION; MICROINJECTION; ZYGOTE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1997
• Issue: 4
• Pages: 71-81

Contributors

author

J. Jura

author

Z. Smorag

author

L. Katska

author

M. Skrzyszowska

author

L-M. Houdebine

author

B. Rynska

• Document Type: article
• Publication order reference: J. Jura, National Research Institute of Animal Production, Department of Animal Reproduction, 32-083 Balice/Krakow, Poland, fax [phone]