PL EN


Preferences help
enabled [disable] Abstract
Number of results
2004 | 45 | 3 | 315-320
Article title

A novel repetitive DNA sequence in lemon [Citrus limon (L.) Burm.] and related species

Title variants
Languages of publication
EN
Abstracts
EN
Repetitive DNA sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of such sequences provide an insight into the organization of the genome of interest. Here, we report the isolation and molecular characterization of a novel repetitive DNA sequence from the genome of Citrus limon. Digestion of C. limon DNA with MboI produced a prominent fragment of approximately 600 bp. Southern blotting revealed a ladder composed of DNA fragments that are multimers of the 600 bp Mbo I band. This suggests that MboI isolated a novel satellite, named C. limon satellite DNA 600 (CL600). Methylation analyses using Sau3AI-MboI isoschizomers demonstrated that most cytosine residues in the GATC sites of this element were methylated in C. limon. This sequence was also found in related citrus plants, like grapefruit and orange, and the hardiest close relatives of Citrus, such as kumquat and trifoliate orange.
Keywords
Discipline
Year
Volume
45
Issue
3
Pages
315-320
Physical description
Contributors
author
author
author
author
References
Document Type
ARTICLE
Publication order reference
B. De Felice, Department of Life Sciences, University of Naples II, Via Vivaldi 43, 81100 Caserta, Italy
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-de51f987-4db0-3dc2-8b8b-7467cce0c795
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.