A novel repetitive DNA sequence in lemon [Citrus limon (L.) Burm.] and related species

• Authors: Felice B. De_ , R.R. Wilson , L. Ciarmiello , C. Conicella
• Languages of publication: EN

Abstracts

EN

Repetitive DNA sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of such sequences provide an insight into the organization of the genome of interest. Here, we report the isolation and molecular characterization of a novel repetitive DNA sequence from the genome of Citrus limon. Digestion of C. limon DNA with MboI produced a prominent fragment of approximately 600 bp. Southern blotting revealed a ladder composed of DNA fragments that are multimers of the 600 bp Mbo I band. This suggests that MboI isolated a novel satellite, named C. limon satellite DNA 600 (CL600). Methylation analyses using Sau3AI-MboI isoschizomers demonstrated that most cytosine residues in the GATC sites of this element were methylated in C. limon. This sequence was also found in related citrus plants, like grapefruit and orange, and the hardiest close relatives of Citrus, such as kumquat and trifoliate orange.

Keywords

EN

CITRUS LIMON; DNA SEQUENCE; METHYLATION

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2004
• Volume: 45
• Issue: 3
• Pages: 315-320

Contributors

author

Felice B. De_

author

R.R. Wilson

author

L. Ciarmiello

author

C. Conicella

• Document Type: ARTICLE
• Publication order reference: B. De Felice, Department of Life Sciences, University of Naples II, Via Vivaldi 43, 81100 Caserta, Italy