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Journal
2002 | 51 | 1-2 | 41-46
Article title

Homologous chromosomes characteristics by sequential banding procedures in rainbow trout Oncorhynchus mykiss

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Languages of publication
EN
Abstracts
EN
The development of high resolution methods of chromosome banding helped the finding of homologous chromosomes, detecting chromosomal abnormalities, and assigning the gene loci to particular chromosomes in mammals. Unfortunately, small and numerous fish chromosomes do not show GC rich and GC poor compartments, this preventing the establishment of G banding pattern. The combination of techniques enabling the identification of constitutive heterochromatin (C-banding), heterochromatin resistant to restriction endonucleas, NOR bearing chromosomes (AgNO3 banding), or AT rich regions on chromosomes (DAPI banding) in sequential staining provides a better characteristic of fish chromosomes. In this work sequentially DAPI, Dde I, AgNO3 stained chromosomes of rainbow trout resulted in the characteristic banding pattern of some homologous chromosomes. Procedure of FISH with telomere probe and DAPI as a counterstaining fluorochrome visualized simultaneous hybridization signals and DAPI banding. Possibility of detection both FISH and DAPI signals can help in procedures of gene mapping on chromosomes.
Publisher

Journal
Year
Volume
51
Issue
1-2
Pages
41-46
Physical description
Contributors
author
author
author
References
Document Type
ARTICLE
Publication order reference
K. Ocalewicz, Department of Evolutionary Genetics, WM University in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-c8c87087-94e0-342c-a6d5-c5f3c34c6b20
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