Homologous chromosomes characteristics by sequential banding procedures in rainbow trout Oncorhynchus mykiss

• Authors: K. Ocalewicz , M. Jakun , M. Luczynski
• Languages of publication: EN

Abstracts

EN

The development of high resolution methods of chromosome banding helped the finding of homologous chromosomes, detecting chromosomal abnormalities, and assigning the gene loci to particular chromosomes in mammals. Unfortunately, small and numerous fish chromosomes do not show GC rich and GC poor compartments, this preventing the establishment of G banding pattern. The combination of techniques enabling the identification of constitutive heterochromatin (C-banding), heterochromatin resistant to restriction endonucleas, NOR bearing chromosomes (AgNO3 banding), or AT rich regions on chromosomes (DAPI banding) in sequential staining provides a better characteristic of fish chromosomes. In this work sequentially DAPI, Dde I, AgNO3 stained chromosomes of rainbow trout resulted in the characteristic banding pattern of some homologous chromosomes. Procedure of FISH with telomere probe and DAPI as a counterstaining fluorochrome visualized simultaneous hybridization signals and DAPI banding. Possibility of detection both FISH and DAPI signals can help in procedures of gene mapping on chromosomes.

Keywords

EN

CHROMOSOME BANDING; GENE MAPPING; HETEROCHROMATIN

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
• Journal: Folia Biologica
• Year: 2002
• Volume: 51
• Issue: 1-2
• Pages: 41-46

Contributors

author

K. Ocalewicz

author

M. Jakun

author

M. Luczynski

• Document Type: ARTICLE
• Publication order reference: K. Ocalewicz, Department of Evolutionary Genetics, WM University in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland