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Journal
2004 | 1 | 284-290
Article title

Mouse recombinant interleukin 2 ? determining optimal conditions of production and activity evaluation in vitro

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PL
Abstracts
EN
Interleukin 2 (IL-2) is the fundamental growth factor for T lymphocytes, both in vivo and in vitro. At our department, specific mouse T lymphocyte clones are used for the examination of antigenic structure of Toxoplasma gondii. Their culturing in vitro requires big amounts of IL-2. That is why we determined optimal conditions for producing crude IL-2 preparation, using recombinant myeloma line X63/0 IL-2 as the producer of IL-2 and cytotoxic lymphocyte line CTL-L as the indicator of IL-2 activity.
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1
Pages
284-290
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author
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ARTICLE
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H. Dlugonska, Katedra Immunologii i Biologii Infekcyjnej, Instytut Mikrobiologii i Immunologii, Wydzial Biologii i Ochrony Srodowiska, Uniwersytet Lodzki, ul. Banacha 12/16, 90-237 Lodz, Poland
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YADDA identifier
bwmeta1.element.element-from-psjc-a9dc0b6a-bf62-34c0-8bca-8ebf22821ec0
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