Mouse recombinant interleukin 2 ? determining optimal conditions of production and activity evaluation in vitro

• Authors: P. Krzyminski , H. Dlugonska
• Languages of publication: PL

Abstracts

EN

Interleukin 2 (IL-2) is the fundamental growth factor for T lymphocytes, both in vivo and in vitro. At our department, specific mouse T lymphocyte clones are used for the examination of antigenic structure of Toxoplasma gondii. Their culturing in vitro requires big amounts of IL-2. That is why we determined optimal conditions for producing crude IL-2 preparation, using recombinant myeloma line X63/0 IL-2 as the producer of IL-2 and cytotoxic lymphocyte line CTL-L as the indicator of IL-2 activity.

Keywords

EN

ACTIVITY; INTERLEUKIN -2; MOUSE; PRODUCTION

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2004
• Issue: 1
• Pages: 284-290

Contributors

author

P. Krzyminski

author

H. Dlugonska

• Document Type: ARTICLE
• Publication order reference: H. Dlugonska, Katedra Immunologii i Biologii Infekcyjnej, Instytut Mikrobiologii i Immunologii, Wydzial Biologii i Ochrony Srodowiska, Uniwersytet Lodzki, ul. Banacha 12/16, 90-237 Lodz, Poland