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Journal
2001 | 4 | 122-134
Article title

Development of an automated yeast identification system by the application of PCR technique: a comparison of inter-line PCR with reference methods

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EN
Abstracts
EN
In the Inter-LINE (IL) PCR method, oligonucleotides GF, GR and 01, which originated from mammalian cells led to highly reproducible patterns of amplified template DNA, based on the consensus of LINE-sequences. These were used for the genomic fingerprinting of about 80 strains of yeast, consisting of 30 species from 13 genera). The IL-PCR technique using the above primers is described and compared to reference methods such as Arbitrarily Primed-PCR and Pulse Field Gel Electrophoresis (PFGE). A comparison of the two PCR variants was performed using suitable numbers of digitized PCR-fingerprints. A database for an automated yeast identification system is proposed.
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Journal
Year
Issue
4
Pages
122-134
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author
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ARTICLE
Publication order reference
A.T. Salek, Milan-Science, Institut f?r Mikrobiologie GmbH & Co., KG, 94036 Passau, Germany
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bwmeta1.element.element-from-psjc-8b5d91c8-99e0-3518-8cbc-c236ee4d09e4
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