Development of an automated yeast identification system by the application of PCR technique: a comparison of inter-line PCR with reference methods

• Authors: A.T. Salek
• Languages of publication: EN

Abstracts

EN

In the Inter-LINE (IL) PCR method, oligonucleotides GF, GR and 01, which originated from mammalian cells led to highly reproducible patterns of amplified template DNA, based on the consensus of LINE-sequences. These were used for the genomic fingerprinting of about 80 strains of yeast, consisting of 30 species from 13 genera). The IL-PCR technique using the above primers is described and compared to reference methods such as Arbitrarily Primed-PCR and Pulse Field Gel Electrophoresis (PFGE). A comparison of the two PCR variants was performed using suitable numbers of digitized PCR-fingerprints. A database for an automated yeast identification system is proposed.

Keywords

EN

IDENTIFICATION; INDUSTRIAL STRAIN; PCR; YEAST

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2001
• Issue: 4
• Pages: 122-134

Contributors

author

A.T. Salek

• Document Type: ARTICLE
• Publication order reference: A.T. Salek, Milan-Science, Institut f?r Mikrobiologie GmbH & Co., KG, 94036 Passau, Germany