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2002 | 43 | 3 | 331-335
Article title

Simultaneous identification of ryanodine receptor 1 (RYR1) and estrogen receptor (ESR) genotypes with the multiplex PCR-RFLP method in Polish Large White and Polish Landrace pigs

Title variants
Languages of publication
EN
Abstracts
EN
A method allowing simultaneous genotyping of two loci: ryanodine receptor 1 (RYR1) and estrogen receptor (ESR) is presented. In multiplex PCR amplification, two amplicons were simultaneously produced: a 272 bp fragment of RYR1 gene and a 185 bp fragment of ESR gene and were then subjected to ?one-tube? restriction enzyme digestion with Hin6 I and Ava I, respectively. A total of 122 Polish Large White and Polish Landrace pigs were genotyped by this method, demonstrating its reliability, convenience and lower costs. This method may be useful in the wide-scale genotyping of both loci in pig breeding programmes.
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Publisher

Year
Volume
43
Issue
3
Pages
331-335
Physical description
Contributors
author
author
author
References
Document Type
SHORT COMMUNICA
Publication order reference
S. Kaminski, University of Warmia and Mazury in Olsztyn, Department of Animal Genetics, ul. Oczapowskiego 5, 10-957 Olsztyn, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-6d7b16ec-8c89-33b4-9e20-10921b9b2dce
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