Simultaneous identification of ryanodine receptor 1 (RYR1) and estrogen receptor (ESR) genotypes with the multiplex PCR-RFLP method in Polish Large White and Polish Landrace pigs

• Authors: S. Kaminska , A. Rusc , K. Wojtasik
• Languages of publication: EN

Abstracts

EN

A method allowing simultaneous genotyping of two loci: ryanodine receptor 1 (RYR1) and estrogen receptor (ESR) is presented. In multiplex PCR amplification, two amplicons were simultaneously produced: a 272 bp fragment of RYR1 gene and a 185 bp fragment of ESR gene and were then subjected to ?one-tube? restriction enzyme digestion with Hin6 I and Ava I, respectively. A total of 122 Polish Large White and Polish Landrace pigs were genotyped by this method, demonstrating its reliability, convenience and lower costs. This method may be useful in the wide-scale genotyping of both loci in pig breeding programmes.

Keywords

EN

ESTROGEN RECEPTOR; GENOTYPING; PCR-RFLP; PIG; RECEPTOR; RYANODINE RECEPTOR

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2002
• Volume: 43
• Issue: 3
• Pages: 331-335

Contributors

author

S. Kaminska

author

A. Rusc

author

K. Wojtasik

• Document Type: SHORT COMMUNICA
• Publication order reference: S. Kaminski, University of Warmia and Mazury in Olsztyn, Department of Animal Genetics, ul. Oczapowskiego 5, 10-957 Olsztyn, Poland