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Number of results

Journal

2007 | 3 | 98-122

Article title

Production of adenoviral vectors of first generation ? optimization of method

Title variants

Languages of publication

PL

Abstracts

EN
The paper presents the production of adenoviral vectors (AdV) containing beta-galactosidase (Adbeta-gal), from the transfer of recombinant viral DNA into packing cell line (HEK293) to the titration of viral particles. Optimisation of the methods (preparation of DNA for transfection, adsorption time during the infection of cells, amount of serum in the medium, time-point of vector isolation) enables obtaining a titer of up to 1010 IU/mL. Adbeta-gal were titrated with several methods, with beta-gal in situ staining used as a reference. We found that the most suitable titration method of the vectors containing other than reporter genes was the Rapid Titer ELISA kit?.

Journal

Year

Issue

3

Pages

98-122

Physical description

Contributors

author
author
author
author
author

References

Document Type

ARTICLE

Publication order reference

Alicja Jozkowicz, Zaklad Biotechnologii Medycznej, Wydzial Biochemii, Biofizyki i Biotechnologii, Uniwersytet Jagiellonski, ul. Gronostajowa 7, 31-271 Krakow, Poland

Identifiers

YADDA identifier

bwmeta1.element.element-from-psjc-691c82bd-3459-3a2c-b3f9-fd43673cd444
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