Production of adenoviral vectors of first generation ? optimization of method

• Authors: M. Stopa , A. Jozwa , J. Mleczko , A. Dulak , A. Jozkowicz
• Languages of publication: PL

Abstracts

EN

The paper presents the production of adenoviral vectors (AdV) containing beta-galactosidase (Adbeta-gal), from the transfer of recombinant viral DNA into packing cell line (HEK293) to the titration of viral particles. Optimisation of the methods (preparation of DNA for transfection, adsorption time during the infection of cells, amount of serum in the medium, time-point of vector isolation) enables obtaining a titer of up to 1010 IU/mL. Adbeta-gal were titrated with several methods, with beta-gal in situ staining used as a reference. We found that the most suitable titration method of the vectors containing other than reporter genes was the Rapid Titer ELISA kit?.

Keywords

EN

ADENOVIRAL VECTOR; ADENOVIRUS; GENE THERAPY

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2007
• Issue: 3
• Pages: 98-122

Contributors

author

M. Stopa

author

A. Jozwa

author

J. Mleczko

author

A. Dulak

author

A. Jozkowicz

• Document Type: ARTICLE
• Publication order reference: Alicja Jozkowicz, Zaklad Biotechnologii Medycznej, Wydzial Biochemii, Biofizyki i Biotechnologii, Uniwersytet Jagiellonski, ul. Gronostajowa 7, 31-271 Krakow, Poland