PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2010 | 58 | 1-2 | 15-20
Article title

Induction of DNA breakage in U937 cells by oxazaphosphorines

Title variants
Languages of publication
EN
Abstracts
EN
Oxazaphosphorines are a class of DNA alkylating agents. The aim of the present study was to compare the possible influence of three new generation oxazaphosphorines, D-17272 (mafosfamide cyclohexylamine salt), D-18864 (4-hydro-peroxy-cyclophosphamide), and D-19575 (glufosfamide, beta-D-glucose-isophosphoramide mustard) on DNA damage induction in the human histiocytic lymphoma U937 cells. The flow cytometry APO-BRDUTM assay, based on the TUNEL method, was used for the in situ detection of DNA strand breaks. After exposure of U937 cells to the oxazaphosphorines, the patterns of temporary changes in the frequency of TUNEL positive U937 cells, expressing DNA breakage, were determined. The effects of the oxazaphosphorines on U937 cells were dependent on the agent tested and its dose, and the time intervals after the drug application. The different potential of D-17272, D-18864 and D-19575 to induce DNA strand breakage in the human histiocytic lymphoma U937 cells was shown.
Publisher

Journal
Year
Volume
58
Issue
1-2
Pages
15-20
Physical description
Contributors
author
author
author
author
References
Document Type
ARTICLE
Publication order reference
Lidia Mazur, Department of Experimental Hematology, Jagiellonian University, R. Ingardena 6, Krakow, Poland
Identifiers
YADDA identifier
bwmeta1.element.element-from-psjc-6240b7ca-7ef7-38b0-a438-10aa9407ea9a
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.