Induction of DNA breakage in U937 cells by oxazaphosphorines

• Authors: L. Mazur , M. Opydo-Chanek , M. Stojak , J. Baran , U. Niemeyer
• Languages of publication: EN

Abstracts

EN

Oxazaphosphorines are a class of DNA alkylating agents. The aim of the present study was to compare the possible influence of three new generation oxazaphosphorines, D-17272 (mafosfamide cyclohexylamine salt), D-18864 (4-hydro-peroxy-cyclophosphamide), and D-19575 (glufosfamide, beta-D-glucose-isophosphoramide mustard) on DNA damage induction in the human histiocytic lymphoma U937 cells. The flow cytometry APO-BRDUTM assay, based on the TUNEL method, was used for the in situ detection of DNA strand breaks. After exposure of U937 cells to the oxazaphosphorines, the patterns of temporary changes in the frequency of TUNEL positive U937 cells, expressing DNA breakage, were determined. The effects of the oxazaphosphorines on U937 cells were dependent on the agent tested and its dose, and the time intervals after the drug application. The different potential of D-17272, D-18864 and D-19575 to induce DNA strand breakage in the human histiocytic lymphoma U937 cells was shown.

Keywords

EN

DNA STRAND BREAK; OXAZAPHOSPHORINES; U937 CELL

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
• Journal: Folia Biologica
• Year: 2010
• Volume: 58
• Issue: 1-2
• Pages: 15-20

Contributors

author

L. Mazur

author

M. Opydo-Chanek

author

M. Stojak

author

J. Baran

author

U. Niemeyer

• Document Type: ARTICLE
• Publication order reference: Lidia Mazur, Department of Experimental Hematology, Jagiellonian University, R. Ingardena 6, Krakow, Poland