PCR-RFLPs within the lactate dehydrogenase (LDH-A) gene of the domestic pigeon (Columba livia var. domestica)
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A total of 45 racing pigeons were genotyped using PCR-RFLP method. PCR product of the LDH-A gene was amplified according to the Long-PCR procedure. The amplification products were digested with restriction enzymes. PCR-RFLPs for two restriction enzymes, HaeIII and NlaIV, were observed. Two pairs of alleles LDH-AA and LDH-AB for LDH-A-NlaIV polymorphism and LDH-AC and LDH-AD for LDH-A-HaeIII polymorphism were detected in the homozygous and heterozygous states. Frequencies of alleles were as follows: A ? 0.622, B ? 0.378 and C ? 0.256, D ? 0.744.
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A. Dybus, Department of Genetics and Animal Breeding, Agricultural University of Szczecin, ul. Doktora Judyma 6, 71-460 Szczecin, Poland