PCR-RFLPs within the lactate dehydrogenase (LDH-A) gene of the domestic pigeon (Columba livia var. domestica)

• Authors: A. Dybus , M. Kmiec
• Languages of publication: EN

Abstracts

EN

A total of 45 racing pigeons were genotyped using PCR-RFLP method. PCR product of the LDH-A gene was amplified according to the Long-PCR procedure. The amplification products were digested with restriction enzymes. PCR-RFLPs for two restriction enzymes, HaeIII and NlaIV, were observed. Two pairs of alleles LDH-AA and LDH-AB for LDH-A-NlaIV polymorphism and LDH-AC and LDH-AD for LDH-A-HaeIII polymorphism were detected in the homozygous and heterozygous states. Frequencies of alleles were as follows: A ? 0.622, B ? 0.378 and C ? 0.256, D ? 0.744.

Keywords

EN

COLUMBA LIVIA; LACTATE DEHYDROGENASE GENE; PCR-RFLP; PIGEON

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2002
• Volume: 43
• Issue: 4
• Pages: 501-504

Contributors

author

A. Dybus

author

M. Kmiec

• Document Type: SHORT COMMUNICA
• Publication order reference: A. Dybus, Department of Genetics and Animal Breeding, Agricultural University of Szczecin, ul. Doktora Judyma 6, 71-460 Szczecin, Poland