PL EN


Preferences help
enabled [disable] Abstract
Number of results
1996 | 56 | 2 | 507-513
Article title

Spingosine stimulates calcium mobilization and modulates calcium signals evoked by thapsigargin in glioma C6 cells

Content
Title variants
Languages of publication
EN
Abstracts
EN
The effect of sphingosine on intracullular calcium signalling in glioma C6 cells was studied with Fura-2 video imaging technique.Sphingosine had a direct effect on changes in cytosolic Ca2+ concentration only when applied at high concentation of 100 muM, causing the cytosolic Ca2+ level to rise.However, at a much lower concentration of 15 muM sphingosine diminished calcium responses triggered by thapsigargin and ionomycin (calcium ionophore).Since responses to thapsigargin and ionomycin were blocked in Ca2+ -free medium, we postulate that sphingosine is acting on the intracellular calcium stores.Additionally, sphingosine (at 15muM and 100muM)markedly decreases thapsigargin-induced sustained elevation in cytosolic Ca2+ concentation, indicating its inhibitory effect in thapsigargin-evoked Ca2+ influx.Sphingosine is known inhibitor of protein kinase C and the involvment of this enzyme is postulated in the modulatory effects of spingosine on intracellular calcium dynamics.
References
Document Type
article
Publication order reference
J.Baranska
YADDA identifier
bwmeta1.element.element-from-psjc-43532ee1-f841-363c-9f47-24585b4066cd
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.