Spingosine stimulates calcium mobilization and modulates calcium signals evoked by thapsigargin in glioma C6 cells

• Authors: P. Sabala , M. Wiktorek , M. Czarny , W. Chaban , J. Baranska
• Languages of publication: EN

Abstracts

EN

The effect of sphingosine on intracullular calcium signalling in glioma C6 cells was studied with Fura-2 video imaging technique.Sphingosine had a direct effect on changes in cytosolic Ca2+ concentration only when applied at high concentation of 100 muM, causing the cytosolic Ca2+ level to rise.However, at a much lower concentration of 15 muM sphingosine diminished calcium responses triggered by thapsigargin and ionomycin (calcium ionophore).Since responses to thapsigargin and ionomycin were blocked in Ca2+ -free medium, we postulate that sphingosine is acting on the intracellular calcium stores.Additionally, sphingosine (at 15muM and 100muM)markedly decreases thapsigargin-induced sustained elevation in cytosolic Ca2+ concentation, indicating its inhibitory effect in thapsigargin-evoked Ca2+ influx.Sphingosine is known inhibitor of protein kinase C and the involvment of this enzyme is postulated in the modulatory effects of spingosine on intracellular calcium dynamics.

Keywords

EN

CALCIUM SIGNAL; GLIOMA C6 CELL; SPINGOSINE; THAPSIGARGIN

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 1996
• Volume: 56
• Issue: 2
• Pages: 507-513

Contributors

author

P. Sabala

author

M. Wiktorek

author

M. Czarny

author

W. Chaban

author

J. Baranska

• Document Type: article
• Publication order reference: J.Baranska