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Journal
2003 | 2 | 268-279
Article title

Beta-galactosidase activity of proteins extracted from Sulfolobus shibatae

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PL
Abstracts
EN
The cytoplasmic -beta galactosidase/beta-glucosidase from hyperthermophilic archaeon Sulfolobus shibatae has been characterized with regard to its use in lactose hydrolysis. Cell extract was purified 16-fold to a specific activity 29.5 U/mg using ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration. Isolated enzyme exhibited optimum activity at pH 5.5 and 98C and had a half-life of 7 h in acetate buffer (pH 5.5) at 90C. Cu2+, Hg2+ and Zn2+ strongly inhibited the enzyme, whereas catalytic properties of other investigated cations were barely influenced. Glucose and galactose were predominantly produced from lactose. However, at the substrate concentration of 0.15, M small amount of lactose was converted into transgalactosylation products.
Journal
Year
Issue
2
Pages
268-279
Physical description
References
Document Type
ARTICLE
Publication order reference
J. Synowiecki, Katedra Chemii i Technologii Zywnosci, Wydzial Chemiczny, Politechnika Gdanska, ul. Gabriela Narutowicza 11/12, 80-952, Gdansk, Poland
YADDA identifier
bwmeta1.element.element-from-psjc-034f2ba5-af3b-34d9-9f8c-35d077fb4878
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