Beta-galactosidase activity of proteins extracted from Sulfolobus shibatae

• Authors: S. Wolosowska , J. Synowiecki
• Languages of publication: PL

Abstracts

EN

The cytoplasmic -beta galactosidase/beta-glucosidase from hyperthermophilic archaeon Sulfolobus shibatae has been characterized with regard to its use in lactose hydrolysis. Cell extract was purified 16-fold to a specific activity 29.5 U/mg using ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration. Isolated enzyme exhibited optimum activity at pH 5.5 and 98C and had a half-life of 7 h in acetate buffer (pH 5.5) at 90C. Cu2+, Hg2+ and Zn2+ strongly inhibited the enzyme, whereas catalytic properties of other investigated cations were barely influenced. Glucose and galactose were predominantly produced from lactose. However, at the substrate concentration of 0.15, M small amount of lactose was converted into transgalactosylation products.

Keywords

EN

BETA-GALACTOSIDASE; SULFOLOBUS SHIBATAE; THERMOSTABLE ENZYME

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2003
• Issue: 2
• Pages: 268-279

Contributors

author

S. Wolosowska

author

J. Synowiecki

• Document Type: ARTICLE
• Publication order reference: J. Synowiecki, Katedra Chemii i Technologii Zywnosci, Wydzial Chemiczny, Politechnika Gdanska, ul. Gabriela Narutowicza 11/12, 80-952, Gdansk, Poland