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2016 | 129 | 2 | 226-228
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NMR Relaxation Measurements as a Tool for Observation of Oxidative Processes

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Water proton relaxation times, T₁ and T₂, were measured to assess the kinetics of the oxidative processes in biological samples. The oxidation in aqueous solutions of albumins was promoted by an addition of 3% hydrogen peroxide (H₂O₂). Immediately following this addition a sharp exponential decrease of both relaxation times was observed. As we confirmed experimentally, the time course of relaxation depended on several essential factors like structure and the concentration of proteins and also the presence of antioxidants added to solution. In experiments with protein solutions containing a small amount of ascorbic acid, after reaching a minimum, relaxation time increased towards the initial (pre-addition H₂O₂) values. We conclude that this T₁ and T₂ recovery is a consequence of the presence of antioxidants and may be used to evaluate its action. This study demonstrates that nuclear magnetic resonance (NMR) relaxation measurements may be useful in evaluating free radicals reactions and antioxidants capacity.
  • Institute of Physics, Pedagogical University, Podchorążych 2, 30-084 Kraków, Poland
  • Institute of Physics, Jagiellonian University, S. Łojasiewicza 11, 30-348 Kraków, Poland
  • Institute of Physics, Jagiellonian University, S. Łojasiewicza 11, 30-348 Kraków, Poland
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