NMR Relaxation Measurements as a Tool for Observation of Oxidative Processes

• Authors: D. Wierzuchowska , L. Skórski , B. Blicharska
• Languages of publication: EN

Abstracts

EN

Water proton relaxation times, T₁ and T₂, were measured to assess the kinetics of the oxidative processes in biological samples. The oxidation in aqueous solutions of albumins was promoted by an addition of 3% hydrogen peroxide (H₂O₂). Immediately following this addition a sharp exponential decrease of both relaxation times was observed. As we confirmed experimentally, the time course of relaxation depended on several essential factors like structure and the concentration of proteins and also the presence of antioxidants added to solution. In experiments with protein solutions containing a small amount of ascorbic acid, after reaching a minimum, relaxation time increased towards the initial (pre-addition H₂O₂) values. We conclude that this T₁ and T₂ recovery is a consequence of the presence of antioxidants and may be used to evaluate its action. This study demonstrates that nuclear magnetic resonance (NMR) relaxation measurements may be useful in evaluating free radicals reactions and antioxidants capacity.

Keywords

EN

76.60.-k; 76.90.+d; 76.30.Rn

Discipline

• 76.60.-k: Nuclear magnetic resonance and relaxation(see also 33.25.+k Nuclear resonance and relaxation in atomic and molecular physics and 82.56.-b Nuclear magnetic resonance in physical chemistry and chemical physics; for structure determination using magnetic resonance techniques, see 61.05.Qr; for biophysical applications, see 87.80.Lg; for NMR in superconducting materials, see 74.25.nj)
• 76.30.Rn: Free radicals
• 76.90.+d: Other topics in magnetic resonances and relaxations (restricted to new topics in section 76)

• Publisher: Institute of Physics, Polish Academy of Sciences
• Journal: Acta Physica Polonica A
• Year: 2016
• Volume: 129
• Issue: 2
• Pages: 226-228

Dates

published

2016-02

Contributors

author

D. Wierzuchowska

• Institute of Physics, Pedagogical University, Podchorążych 2, 30-084 Kraków, Poland

author

L. Skórski

• Institute of Physics, Jagiellonian University, S. Łojasiewicza 11, 30-348 Kraków, Poland

author

B. Blicharska

• Institute of Physics, Jagiellonian University, S. Łojasiewicza 11, 30-348 Kraków, Poland

References

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Identifiers

DOI

10.12693/APhysPolA.129.226