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2018 | 65 | 2 | 259-267
Article title

Protamine-like proteins' analysis as an emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)

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Here we report the industrial pollution effects due to cadmium on the reproductive health of Mytilus galloprovincialis. Mussels were removed from the biofouling of a Conatex panel after one year exposition at a polluted site near a disposal metallurgical factory. A high cadmium bioaccumulation was observed in the testis of mussels housed at the polluted site, with respect to a control site, as determined by inductively coupled plasma-mass spectrometry, along with a 10 fold increase in metallothionein 20 kDa gene (mt20) expression levels determined by qPCR. Furthermore, mussels transferred into laboratory tanks from the reference site, and exposed to 1.5, 5 and 10 µM CdCl2, revealed a 1.7, 3.2 and 4.5 fold expression increase in the testis mt20, respectively, and a positive correlation with cadmium bioaccumulation was found. To evaluate a potential detrimental risk of such alterations on spermatozoa, we carried out electrophoretic analyses on their protamine-like proteins. As determined by AU-PAGE, after 1.5 µM CdCl2 exposure, protamine-like proteins also display major alterations with respect to those obtained after 5 and 10 µM CdCl2 exposure. All protamine-like proteins isolated from the polluted biofouling were in an aggregated form and displayed the same reduced DNA binding affinity of the protamine-like proteins obtained after 1.5 µM CdCl2 as demonstrated EMSA with sperm genomic DNA. Our results contribute to the studies concerning cadmium induced testis alterations and highlight protamine-like proteins' analysis as an emerging biotechnique for cadmium impact assessment on Mytilus galloprovincialis, for the sensitivity of the in vivo and in vitro changes of protamine-like proteins' state and their DNA binding affinity.
Physical description
  • Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy
  • Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Napoli, Italy
  • Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy
  • Department of Biology, El Tarf University, El Tarf, Algeria
  • Theoreo srl-spin-off dell'Università degli Studi di Salerno, 84090 Salerno, Italy
  • Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Napoli, Italy
  • Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy
  • Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy
  • Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy
  • Dipartimento di Scienze Biologiche, Geologiche ed Ambientali, Università di Catania. Catania, Italy
  • Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy
  • Department of Biochemistry & Microbiology, University of Victoria. Victoria, British Columbia, Canada
  • Department of Biochemistry & Microbiology, University of Victoria. Victoria, British Columbia, Canada
  • Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy
  • Interdepartmental Research Center for Environment (I.R.C.Env.), Università degli Studi di Napoli Federico II. Napoli, Italy
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