Protamine-like proteins' analysis as an emerging biotechnique for cadmium impact assessment on male mollusk Mytilus galloprovincialis (Lamarck 1819)

• Authors: Marina Piscopo , Marco Trifuoggi , Rosaria Notariale , Sofiane Labar , Jacopo Troisi , Antonella Giarra , Dea Rabbito , Raffaela Puoti , Domenico de Benedictis , Maria Brundo , Adriana Basile , Katrina Good , Juan Ausió , Giulia Guerriero
• Languages of publication: EN

Abstracts

EN

Here we report the industrial pollution effects due to cadmium on the reproductive health of Mytilus galloprovincialis. Mussels were removed from the biofouling of a Conatex panel after one year exposition at a polluted site near a disposal metallurgical factory. A high cadmium bioaccumulation was observed in the testis of mussels housed at the polluted site, with respect to a control site, as determined by inductively coupled plasma-mass spectrometry, along with a 10 fold increase in metallothionein 20 kDa gene (mt20) expression levels determined by qPCR. Furthermore, mussels transferred into laboratory tanks from the reference site, and exposed to 1.5, 5 and 10 µM CdCl2, revealed a 1.7, 3.2 and 4.5 fold expression increase in the testis mt20, respectively, and a positive correlation with cadmium bioaccumulation was found. To evaluate a potential detrimental risk of such alterations on spermatozoa, we carried out electrophoretic analyses on their protamine-like proteins. As determined by AU-PAGE, after 1.5 µM CdCl2 exposure, protamine-like proteins also display major alterations with respect to those obtained after 5 and 10 µM CdCl2 exposure. All protamine-like proteins isolated from the polluted biofouling were in an aggregated form and displayed the same reduced DNA binding affinity of the protamine-like proteins obtained after 1.5 µM CdCl2 as demonstrated EMSA with sperm genomic DNA. Our results contribute to the studies concerning cadmium induced testis alterations and highlight protamine-like proteins' analysis as an emerging biotechnique for cadmium impact assessment on Mytilus galloprovincialis, for the sensitivity of the in vivo and in vitro changes of protamine-like proteins' state and their DNA binding affinity.

Keywords

EN

Mytilus galloprovincialis; cadmium impact assessment; emerging biotechniques; testis metallothionein; Protamine-like-proteins; spermatozoa

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2018
• Volume: 65
• Issue: 2
• Pages: 259-267

Dates

published

2018

received

2017-11-24

revised

2018-05-14

accepted

2018-05-28

(unknown)

2018-06-15

Contributors

author

Marina Piscopo

• Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy

author

Marco Trifuoggi

• Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Napoli, Italy

author

Rosaria Notariale

• Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy

author

Sofiane Labar

• Department of Biology, El Tarf University, El Tarf, Algeria

author

Jacopo Troisi

• Theoreo srl-spin-off dell'Università degli Studi di Salerno, 84090 Salerno, Italy

author

Antonella Giarra

• Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Napoli, Italy

author

Dea Rabbito

• Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy

author

Raffaela Puoti

• Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy

author

Domenico de Benedictis

• Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy

author

Maria Brundo

• Dipartimento di Scienze Biologiche, Geologiche ed Ambientali, Università di Catania. Catania, Italy

author

Adriana Basile

• Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy

author

Katrina Good

• Department of Biochemistry & Microbiology, University of Victoria. Victoria, British Columbia, Canada

author

Juan Ausió

• Department of Biochemistry & Microbiology, University of Victoria. Victoria, British Columbia, Canada

author

Giulia Guerriero

• Dipartimento di Biologia, Università degli Studi di Napoli Federico II. Napoli, Italy
• Interdepartmental Research Center for Environment (I.R.C.Env.), Università degli Studi di Napoli Federico II. Napoli, Italy

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Identifiers

DOI

10.18388/abp.2017_2533