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2006 | 53 | 3 | 577-584
Article title

pH Profile of cytochrome c-catalyzed tyrosine nitration

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EN
Abstracts
EN
In the present study, we investigated how cytochrome c catalyzed the nitration of tyrosine at various pHs. The cytochrome c-catalyzed nitration of tyrosine occurred in proportion to the concentration of hydrogen peroxide, nitrite or cytochrome c. Thecytochromec-catalyzed nitration of tyrosine was inhibited by catalase, sodium azide, cystein, and uric acid. These results show that the cytochrome c-catalyzednitrotyrosine formation was due to peroxidase activity. The rate constant between cytochrome c and hydrogen peroxide within the pH range of 3 - 8 was the largest at pH 6 (37°C). The amount of nitrotyrosine formed was the greatest at pH 5. At pH 3, onlycytochromec-independent nitration of tyrosine occurred in the presence of nitrite. At this pH, the UV as well as visible spectrum of cytochrome c was changed by nitrite, even in the presence of hydrogen peroxide, probably via the formation of a heme iron - nitric oxide complex. Due to this change, the peroxidase activity of cytochrome c was lost.
Year
Volume
53
Issue
3
Pages
577-584
Physical description
Dates
published
2006
received
2006-05-01
revised
2006-06-21
accepted
2006-08-21
(unknown)
2006-09-04
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv53p577kz
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