pH Profile of cytochrome c-catalyzed tyrosine nitration

• Authors: Yasuhiro Kambayashi , Yoshiaki Hitomi , Norio Kodama , Masayuki Kubo , Junna Okuda , Kei Takemoto , Masafumi Shibamori , Tomoko Takigawa , Keiki Ogino
• Languages of publication: EN

Abstracts

EN

In the present study, we investigated how cytochrome c catalyzed the nitration of tyrosine at various pHs. The cytochrome c-catalyzed nitration of tyrosine occurred in proportion to the concentration of hydrogen peroxide, nitrite or cytochrome c. Thecytochromec-catalyzed nitration of tyrosine was inhibited by catalase, sodium azide, cystein, and uric acid. These results show that the cytochrome c-catalyzednitrotyrosine formation was due to peroxidase activity. The rate constant between cytochrome c and hydrogen peroxide within the pH range of 3 - 8 was the largest at pH 6 (37°C). The amount of nitrotyrosine formed was the greatest at pH 5. At pH 3, onlycytochromec-independent nitration of tyrosine occurred in the presence of nitrite. At this pH, the UV as well as visible spectrum of cytochrome c was changed by nitrite, even in the presence of hydrogen peroxide, probably via the formation of a heme iron - nitric oxide complex. Due to this change, the peroxidase activity of cytochrome c was lost.

Keywords

EN

pseudo-peroxidase; pH; cytochrome c; nitrite; nitrotyrosine

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2006
• Volume: 53
• Issue: 3
• Pages: 577-584

Dates

published

2006

received

2006-05-01

revised

2006-06-21

accepted

2006-08-21

(unknown)

2006-09-04

Contributors

author

Yasuhiro Kambayashi

• Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan

author

Yoshiaki Hitomi

• Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan

author

Norio Kodama

• Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan

author

Masayuki Kubo

• Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan

author

Junna Okuda

• Department of Environmental and Preventive Medicine, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan

author

Kei Takemoto

• Department of Public Health, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan

author

Masafumi Shibamori

• Third Institute of New Drug Discovery, Otsuka Pharmaceutical Co., Ltd., Tokushima, Japan

author

Tomoko Takigawa

• Department of Public Health, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan

author

Keiki Ogino

• Department of Public Health, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama, Japan

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