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2003 | 50 | 4 | 1195-1203
Article title

Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.

Content
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EN
Abstracts
EN
Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise measurement of allelic ratios. We examined 70 breast cancer and 70 control DNA specimens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.
Publisher

Year
Volume
50
Issue
4
Pages
1195-1203
Physical description
Dates
published
2003
revised
2003-07-09
accepted
2003-08-18
received
2003-12-10
Contributors
  • Department of Human Biology, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland
  • Forensic Medicine Institute, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland
author
  • Forensic Medicine Institute, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland
  • Forensic Medicine Institute, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland
  • Forensic Medicine Institute, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland
author
  • Department of Human Biology, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland
  • Regional Center of Oncology, Bydgoszcz, Poland
author
  • Regional Center of Oncology, Bydgoszcz, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.bwnjournal-article-abpv50i4p1195kz
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