Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.

• Authors: Jolanta Powierska-Czarny , Danuta Miścicka-Śliwka , Jakub Czarny , Tomasz Grzybowski , Marcin Woźniak , Gerard Drewa , Włodzimierz Czechowicz , Jan Sir
• Languages of publication: EN

Abstracts

EN

Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise measurement of allelic ratios. We examined 70 breast cancer and 70 control DNA specimens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.

Keywords

EN

microsatellite instability; short tandem repeats; multiplex DNA typing; breast cancer

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2003
• Volume: 50
• Issue: 4
• Pages: 1195-1203

Dates

published

2003

revised

2003-07-09

accepted

2003-08-18

received

2003-12-10

Contributors

author

Jolanta Powierska-Czarny

• Department of Human Biology, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland

author

Danuta Miścicka-Śliwka

• Forensic Medicine Institute, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland

author

Jakub Czarny

• Forensic Medicine Institute, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland

author

Tomasz Grzybowski

• Forensic Medicine Institute, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland

author

Marcin Woźniak

• Forensic Medicine Institute, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland

author

Gerard Drewa

• Department of Human Biology, The Ludwik Rydygier Medical University of Bydgoszcz, Bydgoszcz, Poland

author

Włodzimierz Czechowicz

• Regional Center of Oncology, Bydgoszcz, Poland

author

Jan Sir

• Regional Center of Oncology, Bydgoszcz, Poland

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