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Genetic variability of potato spindle tuber viroid RNA replicon.

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The genetic continuity of the potato spindle tuber viroid (PSTVd) genome was analysed after infection of tomato plants with cloned cDNAs of parental strains. During the six weeks of the experiment, several new sequence variants appeared. The sequence variants detected in the progeny population induced sequence-specific disease symptoms. The PSTVd genome therefore follows the pattern expected for typical pseudo-strains propagating in plants as a population of similar sequences. Assessing further the replicon continuity, a PSTVd cDNA mutant with a deletion in the central conserved region was constructed and proven to be non-infectious. Surprisingly, in a sub-population of potato transformants expressing the same deleted PSTVd RNA an infectious viroid was detected. This suggests specific transcript conversion followed by recovery of the full-length pathogen genome.
Physical description
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
  • Virologie Végétale, UMR GD2P, IBVM, INRA, BP81, Villenave d'Ornon, Cedex, France
  • Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland
  • Baumstark, T., Schröder, A.R.W. & Riesner, D. (1997) Viroid processing: Switch from cleavage to ligation is driven by a change from a tetraloop to a loop E conformation. EMBO J. 16, 599-610.
  • Camacho Henriquez, A. & Sänger, H.L. (1982a) Gel electrophoretic analysis of phenol-extractable leaf proteins from different viroid/host combinations. Arch. Virol. 74, 167-180.
  • Camacho Henriquez, A. & Sänger, H.L. (1982b) Analysis of acid-extractable tomato leaf proteins after infection with a viroid, two viruses and a fungus and partial purification of the pathogenesis-related protein p14. Arch. Virol. 74, 181-196.
  • Candresse, T., Diener, T.O. & Owens, R.A. (1990) The role of the viroid central conserved region in cDNA infectivity. Virology 175, 232-237.
  • Chrzanowska, M., Kowalska-Noordam, A., Zagórska, H. & Skrzeczkowska, S. (1984) Reakcja polskich odmian ziemniaka na silny szczep wiroida wrzecionowatości bulw. Biuletyn Instytutu Ziemniaka 31, 15-27 (in Polish).
  • Conejero, V., Picazo, I. & Segado, P. (1979) Citrus exocortis viroid (CEV): Protein alternations in different hosts following viroid infection. Virology 97, 454-456.
  • Conejero, V. & Semancik, J.S. (1977) Exocortis viroid: Alternation in the proteins of Gynura aurantiaca accompanying viroid infection. Virology 77, 221-232.
  • Cress, D.E., Kiefer, M.C. & Owens, R.A. (1983) Construction of infectious potato spindle tuber viroid cDNA clones. Nucleic Acids Res. 11, 6821-6835.
  • Diener, T.O. (1971) Potato spindle tuber virus. IV. A replicating, low molecular weight RNA. Virology 45, 411-428.
  • Diener, T.O. (1986) Viroid processing: A model involving the central conserved region and hairpin I. Proc. Natl. Acad. Sci. U.S.A. 83, 58-62.
  • Diener, T.O. (1987) Potato spindle tuber (diagnosis); in The Viroids (Diener, T.O., ed.) pp. 228-230, Plenum Press, New York.
  • Eigen, M. (1993) The origin of genetic information: Viruses as models. Gene 135, 37-47.
  • Eigen, M. & Winkler-Oswatitsch, R. (1990) Statistical geometry on sequence space. Methods Enzymol. 183, 505-530.
  • Góra, A., Candresse, T. & Zagórski, W. (1994) Analysis of the population structure of three phenotypically different PSTVd isolates. Arch. Virol. 138, 233-245.
  • Góra-Sochacka, A., Kierzek, A., Candresse, T. & Zagórski, W. (1997) The genetic stability of potato spindle tuber viroid (PSTVd) molecular variants. RNA 3, 68-74.
  • Góra-Sochacka, A., Rosa, I., Podstolski, W., Candresse, T. & Zagórski, W. (2000) Transgenic potato plants carrying truncated cDNA copy of a severe strain of PSTVd; in Use of Agriculturally Important Genes in Biotechnology, NATO Science Series A, Life Sciences, IOS Press, 319, 14-18.
  • Gross, H.J., Domdey, H., Lossow, C., Jank, P., Raba, M., Alberty, H. & Sänger, H.L. (1978) Nucleotide sequence and secondary structure of potato spindle tuber viroid. Nature 273, 203-208.
  • Gross, H.J., Liebel, U., Alberty, H., Krupp, G., Domdey, H., Ramm, K. & Sänger, H.L. (1981) A severe and mild potato spindle tuber viroid isolate differ in three nucleotide exchanges only. Biosci. Rep. 1, 235-241.
  • Herold, T., Hass, B., Singh, R.P., Boucher, A. & Sänger, H.L. (1992) Sequence analysis of five new field isolates demonstrates that the chain length of potato spindle tuber viroid (PSTVd) is not strictly conserved but as variable as in other viroids. Plant Mol. Biol. 19, 329-333.
  • Kay, R. & McPherson, J. (1987) Hybrid pUC vectors for addition of new restriction enzyme sites to the ends of DNA fragments. Nucleic Acids Res. 15, 2778.
  • Keese, P. & Symons, R.H. (1985) Domains in viroids: Evidence of intermolecular RNA rearrangements and their contribution to viroid evolution. Proc. Natl. Acad. Sci. U.S.A. 82, 4582-4586.
  • Keese, P. & Symons, R.H. (1987) The structure of viroids and virusoids; in Viroids and Viroid-like Pathogens (Semancik, J.S., ed.) pp. 1-47, CRC Press, Boca Raton, FL.
  • Kowalska-Noordam, A. & Skrzeczkowska, S. (1984) Reakcja roślin ziemniaka rodu PW 22/70 na zakażenie silnym i słabym szczepem PSTV. Biuletyn Instytutu Ziemniaka 31, 29-43 (in Polish).
  • Kowalska-Noordam, A., Chrzanowska, M. & Skrzeczkowska, S. (1986/87) Reaction of ten Polish potato cultivars to severe and mild strains of potato spindle tuber viroid. The Potato, 71-91.
  • Kuo, T.T. (1979) Nucleotide sequence and secondary structure of potato spindle tuber viroid. NTU Phytopathol. Enzymol. 6, 1-9.
  • Lakshman, D.K. & Tavantzis, S.M. (1993) Primary and secondary structure of 360-nucleotide isolate of potato spindle tuber viroid. Arch. Virol. 128, 319-331.
  • Maiti, I.B., Murthy, J.F., Shaw, J.G. & Hunt, A.G. (1993) Plants that express a potyvirus proteinase gene are resistant to virus infection. Proc. Natl. Acad. Sci. U.S.A. 90, 6110-6114.
  • Martini, N., Egen, M., Runtz, I. & Strittmatter, G. (1993) Promoter sequence of potato pathogenesis-related gene mediate transcriptional activation upon fungal infection. Mol. Gen. Genet. 236, 179-186.
  • Owens, R.A., Hammond, R.W., Gardner, R.C., Kiefer, M.C., Thompson, S. & Cress, D.E. (1986) Site specific mutagenesis of potato spindle tuber viroid cDNA. Plant Mol. Biol. 6, 179-192.
  • Owens, R.A., Khurana, S.M., Smith, D.R., Singh, M.N. & Garg, I.D. (1992) A new mild strain of potato spindle tuber viroid isolated from wild Solanum spp. in India. Plant Dis. 76, 527-529.
  • Pfannenstiel, M.A. & Slack, S.A. (1980) Response of potato cultivars to infection by the potato spindle tuber viroid. Phytopathology 70, 922-926.
  • Sänger, H.L. (1987) Viroid function - viroid replication; in The Viroids (Diener, T.O., ed.,) pp. 63-98, Plenum, New York.
  • Schnölzer, M., Hass, B., Ramm, K., Hofmann, H. & Sänger, H.L. (1985) Correlation between structure and pathogenicity of potato spindle tuber viroid (PSTVd). EMBO J. 4, 2181-2190.
  • Steger, G., Baumstark, T., Morchen, M., Tabler, M., Tsagris, M., Sänger, H.L. & Riesner, D. (1992) Structural requirements for viroid processing by RNase T1. J. Mol. Biol. 227, 719-737.
  • Van Wezenbek, P., Vos, P., Van Boom, J. & Van Kammen, A. (1982) Molecular cloning and characterization of a complete DNA copy of potato spindle tuber viroid RNA. Nucleic Acids Res. 10, 7947-7957.
  • Wassenegger, M., Heimes, S. & Sänger, H.L. (1994) An infectious viroid RNA replicon evolved from an in vitro-generated non-infectious viroid deletion mutant via a complementary deletion in vivo. EMBO J. 13, 6172-6177.
  • Zaitlin, M. & Hariharasubramanian, V. (1972) A gel electrophoretic analysis of proteins from plants infected with tobacco mosaic and potato tuber viruses. Virology 47, 296-305.
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