Genetic variability of potato spindle tuber viroid RNA replicon.

• Authors: Anna Góra-Sochacka , Thierry Candresse , Włodzimierz Zagórski
• Languages of publication: EN

Abstracts

EN

The genetic continuity of the potato spindle tuber viroid (PSTVd) genome was analysed after infection of tomato plants with cloned cDNAs of parental strains. During the six weeks of the experiment, several new sequence variants appeared. The sequence variants detected in the progeny population induced sequence-specific disease symptoms. The PSTVd genome therefore follows the pattern expected for typical pseudo-strains propagating in plants as a population of similar sequences. Assessing further the replicon continuity, a PSTVd cDNA mutant with a deletion in the central conserved region was constructed and proven to be non-infectious. Surprisingly, in a sub-population of potato transformants expressing the same deleted PSTVd RNA an infectious viroid was detected. This suggests specific transcript conversion followed by recovery of the full-length pathogen genome.

Keywords

EN

genetic stability; sequence diversity; infectious clones; pathogenicity; potato spindle tuber viroid

• Publisher: Polish Biochemical Society
• Journal: Acta Biochimica Polonica
• Year: 2001
• Volume: 48
• Issue: 2
• Pages: 467-476

Dates

published

2001

received

2001-02-14

revised

2001-05-24

accepted

2001-05-31

Contributors

author

Anna Góra-Sochacka

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

author

Thierry Candresse

• Virologie Végétale, UMR GD2P, IBVM, INRA, BP81, Villenave d'Ornon, Cedex, France

author

Włodzimierz Zagórski

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa, Poland

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