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2012 | 33 | 1 | 71-84
Article title

Impact of bioreactor scale on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture

Content
Title variants
Languages of publication
EN
Abstracts
EN
Biosynthesis of lovastatin (a polyketide metabolite of Aspergillus terreus) in bioreactors of different working volume was studied to indicate how the change of scale of the process influences the formation of this metabolite. The experiments conducted in shake flasks of 150 ml working volume allowed to obtain lovastatin titres at the level of 87.5 mg LOV l-1, when two carbon sources, namely lactose and glycerol were used. The application of the same components in a large stirred-tank bioreactor of 5.3-litre working volume caused a decrease of lovastatin production by 87% compared to the shake flask culture. The deficiency of nitrogen in this bioreactor did not favour the formation of lovastatin, in contrast to the small bioreactor of 1.95-litre working volume, in which lovastatin titres comparable to those in the shake flasks could be achieved, when organic nitrogen concentration was two-fold decreased. When the control of pH and/or pO2 was used simultaneously, an increase in lovastatin production was observed in the bioreactors. However, these results were still slightly lower than lovastatin titres obtained in the shake flasks.
Publisher

Year
Volume
33
Issue
1
Pages
71-84
Physical description
Dates
published
1 - 3 - 2012
online
6 - 3 - 2012
Contributors
author
  • Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Technical University of Lodz, ul. Wólczańska 213, 90-924 Łódź, Poland
  • Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Technical University of Lodz, ul. Wólczańska 213, 90-924 Łódź, Poland
  • Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Technical University of Lodz, ul. Wólczańska 213, 90-924 Łódź, Poland
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_v10176-012-0007-0
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