Impact of bioreactor scale on lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture

• Authors: Marta Pawlak , Marcin Bizukojć , Stanisław Ledakowicz
• Languages of publication: EN

Abstracts

EN

Biosynthesis of lovastatin (a polyketide metabolite of Aspergillus terreus) in bioreactors of different working volume was studied to indicate how the change of scale of the process influences the formation of this metabolite. The experiments conducted in shake flasks of 150 ml working volume allowed to obtain lovastatin titres at the level of 87.5 mg LOV l-1, when two carbon sources, namely lactose and glycerol were used. The application of the same components in a large stirred-tank bioreactor of 5.3-litre working volume caused a decrease of lovastatin production by 87% compared to the shake flask culture. The deficiency of nitrogen in this bioreactor did not favour the formation of lovastatin, in contrast to the small bioreactor of 1.95-litre working volume, in which lovastatin titres comparable to those in the shake flasks could be achieved, when organic nitrogen concentration was two-fold decreased. When the control of pH and/or pO2 was used simultaneously, an increase in lovastatin production was observed in the bioreactors. However, these results were still slightly lower than lovastatin titres obtained in the shake flasks.

Keywords

EN

lovastatin; Aspergillus terreus ATCC 20542; shake flask; stirred tank bioreactor; batch process

• Publisher: De Gruyter Open
• Journal: Chemical and Process Engineering
• Year: 2012
• Volume: 33
• Issue: 1
• Pages: 71-84

Dates

published

1 - 3 - 2012

online

6 - 3 - 2012

Contributors

author

Marta Pawlak

• Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Technical University of Lodz, ul. Wólczańska 213, 90-924 Łódź, Poland

author

Marcin Bizukojć

• Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Technical University of Lodz, ul. Wólczańska 213, 90-924 Łódź, Poland

author

Stanisław Ledakowicz

• Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Technical University of Lodz, ul. Wólczańska 213, 90-924 Łódź, Poland

References

• Bizukojć M., Ledakowicz S., 2007a. A macrokinetic modelling of the biosynthesis of lovastatin by Aspergillus terreus. J. Biotechnol., 130, 422-435. DOI: 10.1016/j.biotec.2007.05.007.[Crossref][WoS]
• Bizukojć M., Ledakowicz S., 2007b. Simultaneous biosynthesis of (+)-geodin by a lovastatin-producing fungus Aspergillus terreus. J. Biotechnol., 132, 453-460. DOI: 10.1016/j.jbiotec.2006.06.017.[WoS][Crossref]
• Bizukojć M., Ledakowicz S., 2008. Biosynthesis of lovastatin and (+)-geodin by Aspergillus terreus in batch and fed-batch culture in the stirred tank bioreactor. Biochem. Eng., 42, 198-207. DOI: 10.1016/j.bej.2008.06.022.[Crossref][WoS]
• Bizukojć M., Pecyna M., 2011. Lovastatin and (+)-geodin formation by Aspergillus terreus ATCC 20542 in a batch culture with the simultaneous use of lactose and glycerol as carbon sources. Eng. Life Sci., 11, 272-282. DOI: 10.1002/elsc.201000179.[Crossref]
• Casas Lopez J. L., Sanchez Perez J. A., Fernandez Sevilla J. M., Acien Fernandez F. G., Molina Grima E., Chisti Y., 2003. Production of lovastatin by Aspergillus terreus: effects of C:N ratio and the principal nutrients on growth and metabolite production. Enzyme Microb. Technol., 33, 270-277. DOI: 10.1016/S0141-0229(03)00130-3.[Crossref]
• Casas Lopez J. L., Sanchez Perez J. A., Fernandez Sevilla J. M., Acien Fernandez F. G., Molina Grima E., Chisti Y., 2004. Fermentation optimization for the production of lovastatin by Aspergillus terreus; use of response surface methodology. J. Chem. Technol. Biotechnol., 79, 1119-1126. DOI: 10.1002/jctb.1100.[Crossref]
• Casas Lopez J. L., Sanchez Perez J. A., Fernandez Sevilla J. M., Rodriguez Porcel E. M., Chisti Y., 2005. Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus. J. Biotechnol., 116, 61-77. DOI: 10.1016/j.jbiotec.2004.10.005.[Crossref]
• Hajjaj H., Niederberger P., Duboc P., 2001. Lovastatin biosynthesis by Aspergillus terreus in a chemically defined medium. Appl. Environ. Microbiol., 67, 2596-2602. DOI: 10.1128/AEM.67.6.2596-2602.2001.[Crossref]
• Kennedy J., Auclair K., Kendrew S. G., Park C., Vederas J., Hutchinson C. R., 1999. Modulation of polyketide synthase activity by accessory proteins during lovastatin biosynthesis. Science, 284, 1368-1372. DOI: 10.1126/science.284.5418.1368.[Crossref]
• Kumar M. S., Jana S. K., Senthil V., Shashanka V., Kumar S. V. Sadhukhan A. K., 2000. Repeated fed-batch process for improving lovastatin production. Proc. Biochem., 36, 363-368. DOI: 10.1016/S0032-9592(00)00222-3.[Crossref]
• Lai L-S. T., Pan C-C., Tzeng B-K., 2002. Medium optimization for lovastatin production by Aspergillus terreus in submerged cultures. J. Chin. Inst. Chem. Engrs., 33, 517-527.
• Lai L-ST., Tsai T-H, Wang T. C., Cheng T. Y., 2005. The influence of culturing environments on lovastatin production by Aspergillus terreus in submerged cultures. Enz. Microb. Technol., 36, 737-748. DOI: 10.1016/j.enzmictec.2004.12.021.[Crossref]
• Manzoni M., Rollini M., Bergomi S., Cavazzoni V., 1998. Production of statinsby filamenous fungi. Biotechnol. Lett., 21, 253-257. DOI: 10.1023/A:1005495714248.[Crossref]
• Rodriguez Porcel E. M., Casas López J. L., Sanchez Pérez J. A., Fernández Sevilla J. M., Garcia Sánchez J. L., Chisti Y., 2006. Aspergillus terreus broth rheology, oxygen transfer, and lovastatin production in a gas-agitated slurry reactor. Ind. Eng. Chem. Res., 45, 4837-4843. DOI: 10.1021/ie0600801.[Crossref]

Identifiers

DOI

10.2478/v10176-012-0007-0