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2012 | 14 | 4 | 57-60
Article title

Controllable preparation of highly active horseradish peroxidase-gold nanoparticle bionanoconjugate

Content
Title variants
Languages of publication
EN
Abstracts
EN
A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications in the fields including preparing highly active bio-nanoprobe and immobilized enzyme.
Publisher

Year
Volume
14
Issue
4
Pages
57-60
Physical description
Dates
published
1 - 12 - 2012
online
12 - 01 - 2013
Contributors
author
  • Jiangnan University, School of Food Science and Technology, State Key Lab of Food Science and Technology, Wuxi, Jiangsu, 214122, PR China
author
  • Jiangnan University, School of Food Science and Technology, State Key Lab of Food Science and Technology, Wuxi, Jiangsu, 214122, PR China
author
  • Jiangnan University, School of Food Science and Technology, State Key Lab of Food Science and Technology, Wuxi, Jiangsu, 214122, PR China
author
  • Jiangnan University, School of Food Science and Technology, State Key Lab of Food Science and Technology, Wuxi, Jiangsu, 214122, PR China, pcf2125@yahoo.com.cn
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_v10026-012-0103-4
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