Controllable preparation of highly active horseradish peroxidase-gold nanoparticle bionanoconjugate

• Authors: Peng Zhang , Chunli Liu , Shanshan Song , Chifang Peng
• Languages of publication: EN

Abstracts

EN

A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications in the fields including preparing highly active bio-nanoprobe and immobilized enzyme.

Keywords

EN

horseradish peroxidase; gold nanoparticles; bionanoconjugate.

• Publisher: De Gruyter Open
• Journal: Polish Journal of Chemical Technology
• Year: 2012
• Volume: 14
• Issue: 4
• Pages: 57-60

Dates

published

1 - 12 - 2012

online

12 - 01 - 2013

Contributors

author

Peng Zhang

• Jiangnan University, School of Food Science and Technology, State Key Lab of Food Science and Technology, Wuxi, Jiangsu, 214122, PR China

author

Chunli Liu

• Jiangnan University, School of Food Science and Technology, State Key Lab of Food Science and Technology, Wuxi, Jiangsu, 214122, PR China

author

Shanshan Song

• Jiangnan University, School of Food Science and Technology, State Key Lab of Food Science and Technology, Wuxi, Jiangsu, 214122, PR China

author

Chifang Peng

• Jiangnan University, School of Food Science and Technology, State Key Lab of Food Science and Technology, Wuxi, Jiangsu, 214122, PR China

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Identifiers

DOI

10.2478/v10026-012-0103-4