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2008 | 10 | 1 | 52-56

Article title

Modeling of the influence of the modifier concentration on the retention process in NP-HPLC


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The adsorption model for an accurate prediction of the analyte retention in the normal - phase liquid chromatography with a binary mobile phase has been proposed. This model was derived using a thermody-namically consistent modified competitive Langmuir isotherm. The performance of the proposed equation was compared with two retention models reported in literature. All models were verified for different NP-LC systems by use of three criteria: the sum of squared differences between the experimental and theoretical data, approximation of the standard deviation and the Fisher test.









Physical description


1 - 1 - 2008
3 - 4 - 2008


  • Department of Chemical and Process Engineering, Chemical Faculty, Rzeszów University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland


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