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2012 | 7 | 6 | 769-774

Article title

Asymptomatic carriers of clostridium difficile in serbian population


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The aim of the research was to determine the intestinal carriers of C. difficile in different human population groups in Serbia. The research enrolled 877 persons with formed stools: (newborn children in maternity hospitals for up to two weeks old) (23), group A; children aged from two weeks to two years (121), group B; children aged two to 10 years (54), group C, healthy individuals aged 10 and over (516), group D; patients hospitalized for at least 48 hours (100), group E; staff of the Clinical Center in Nis, Serbia, (63), group F. The toxins A and B of C. difficile were detected by ELISA-ridascreen Clostridium difficile Toxin A/B (R - Biopharm AG, Darmstadt, Germany). The toxin A of C. difficile was detected using ColorPAC Toxin A test (BectonDickinson, New Jersey, USA). Out of the total number of persons (877), the carriers of certain types of toxin-producing strains of C. difficile were distributed as: 6.04% (A−/B−), 1.83% (A+/B+) and 0.11% (A−/B+). In most groups (5/6), the dominance of non-toxigenic (A−/B−) isolates was established, with the rate of carriers 1.75 – 30.43% depending on the group. Toxigenic isolates were prevalent only in the group F in relation to non - toxigenic (7.94% versus 4.76% of persons). In other groups, the carriers of toxigenic strains ranged from 0.00 – 17.45%. The presence of asymptomatic intestinal carriers of C. difficile in the human population, indicate the possible reservoirs and sources of infection.










Physical description


1 - 12 - 2012
11 - 10 - 2012


  • Gynecology — Obstetrics Clinic (Department of Neonatology), 18000, Nis, Serbia
  • Institute for Public Health Nis, Center of Microbiology, 18000, Nis, Serbia
  • Clinical Centar, 18000, Nis, Serbia


  • [1] Bouza E, Munoz P, Alonso R. Clinical manifestations, tretment and control of infections caused by Clostridium difficile. Clin Microbiol Infec 2005;11(4):57–64 http://dx.doi.org/10.1111/j.1469-0691.2005.01165.x[Crossref]
  • [2] Bolton R, Tait S, Dear P, Losowsky MS. Asymptomatic neonatal colonisation by Clostridium difficile. Arch Dis Child 1984; 59(5):466–72 http://dx.doi.org/10.1136/adc.59.5.466[Crossref]
  • [3] Kato H, Kita H, Karasawa T, Maegawa T, Koino Y, Takakuwa H et al. Colonisation and transmission of Clostridium difficile in healthy individuals examined by ribotiping and pulsed-field gel electrophoresis. J Med Microbiol 2001; 50(8):720–727
  • [4] Simor A, Yeke S, Tsimidis K. Infection due to Clostridium difficile among elderly of a Long-termcare facilities. Clin Infect Dis 1993; 17(4):672–678 http://dx.doi.org/10.1093/clinids/17.4.672[Crossref]
  • [5] Simor A, Bradley S, Strausbaugh LJ, Crossley K, Nicolle L. Clostridium difficile in Long-term-care facilities for the elderly. Infect Contr Hosp Epidem 2002; 23(11):696–703 http://dx.doi.org/10.1086/501997[Crossref]
  • [6] Larson H, Barcley F, Honour P, Hill I. Epidemiology of Clostridium difficile in infants. J Infect Dis 1982; 146(6):727–733 http://dx.doi.org/10.1093/infdis/146.6.727[Crossref]
  • [7] Matsuki S, Ozaki E, Shouzi M, Inoue M, Shimizu S, Yamaguchi N et al. Colonization by Clostridium difficile in a hospital, and infants and children in three day-care facilities of Kanazawa, Japan. Internac Microbiol 2005; 8(1):43–48
  • [8] El Mohandes A, Keiser J, Refat M, Jackson B. Prevalence and toxigenicity of Clostridium difficile isolates in fecal microflora of preterm infants care nursery. Biol Neonate 1993; 63(4):225–229 http://dx.doi.org/10.1159/000243935[Crossref]
  • [9] Riggs M, Seithi A, Zabarsky T, Eckstein E, Jump R, Donskey C. Asymptomatic carriers are a potential source for transmission of epidemic and nonepidemic Clostridium difficile strains among Long-term care facility residents. Clinic Infect Dis 2007; 45(8):992–998 http://dx.doi.org/10.1086/521854[WoS][Crossref]
  • [10] Nakamura S, Mikawa M, Nakashio S, Takabatake M, Okaida I, Yamakawa K et al. Isolation of Clostridium difficile from feces and the antibody in sera of young and elderly adults. Microbiol Immun 1981; 25(4):345–351 [Crossref]
  • [11] Larson H, Price A, Honour P, Borellio S. Clostridium difficile and the aetiology of pseudomembranous colitis. Lancet 1978; 1(8073):1063–1066 http://dx.doi.org/10.1016/S0140-6736(78)90912-1[Crossref]
  • [12] Ryan J, Murphy C, Twomey C, Paul Ross R, Rea M, MacSharry J et al. Asymptomatic carriage of Clostridium difficile in a Irish continuing care institution for the elderl: prevalence and characteristics. Ir J Med Sci 2010; 179(2):245–250 http://dx.doi.org/10.1007/s11845-009-0361-1[WoS][Crossref]
  • [13] Walker K, Gilliland S, Vance-Bryan K, Moody J, Larsson A, Rotschafer J et al. Clostridium difficile in residents of long-term care facilities: prevalence and risk factors. J Am Geriatr Soc 1993; 41(9):940–946 [Crossref]
  • [14] Rudensky B, Rosoner S, Sonnenblick M, van Dijk Y, Shapira E, Isaacsohn M. The prevalence and nosocomial acquisition of Clostridium difficile in elderly hospitalized patients. Postgrad Med J 1993; 69(807):45–47 http://dx.doi.org/10.1136/pgmj.69.807.45[Crossref]
  • [15] McFarland L, Mulligan M, Kwok R, Stamm W. Nosocomial aquisition of Clostridium dificile infection. N Engl J Med 1989; 320(4):204–210 http://dx.doi.org/10.1056/NEJM198901263200402[Crossref]
  • [16] Thibault A, Miller MA, Gaese C. Risk factors for the development of Clostridium diffcile-associated diarrhea during a hospital outbreak. Infect Control Hospital Epidemiol 1991; 12(6):345–348 http://dx.doi.org/10.1086/646354[Crossref]
  • [17] Clabots R, Johnson S, Olson MM, Peterson LR, Gerding DL. Acquisition of Clostridium difficile by hospitalized patients: evidence for colonized new admissions as source of infection. J Infect Dis 1992; 166(3):561–567 http://dx.doi.org/10.1093/infdis/166.3.561[Crossref]
  • [18] Brooks SE, Veal RO, Kramer M, Dore L, Schupf N, Adachi M. Reduction in the incidence of Clostridium diffcile-associated diarrhea in acute care hospital and a skilled nursing facility following replacement of electronic thermometers with single use disposable. Infect Control Hospital Epidemiol 1992; 13(2):98–103 http://dx.doi.org/10.1086/646480[Crossref]
  • [19] Aslam S, Hamill R, Musher D. Treatment of Clostridium difficile-associated disease: Old therapies and new strategies. Lancet Infect Dis 2005; 5(9):549–557 http://dx.doi.org/10.1016/S1473-3099(05)70215-2[Crossref]
  • [20] Kyne L, Warny M, Qamar A, Kelly CP. Association between antibody response to toxin A and protection against recurrent Clostridium difficile diarrhoea. Lancet 2001; 357(9251):189–193 http://dx.doi.org/10.1016/S0140-6736(00)03592-3[Crossref]

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