Pharmacologic responses of the mouse urinary bladder

• Authors: A. Canda , Christopher Chapple , Russ Chess-Williams
• Languages of publication: EN

Abstracts

EN

The aim of the study was to determine pathways involved in contraction and relaxation of the mouse urinary bladder. Mouse bladder strips were set up in gassed Krebs-bicarbonate solution and responses to various drugs and electrical field stimulation were obtained. Isoprenaline (b-receptor agonist) caused a 63% inhibition of carbachol precontracted detrusor (EC50=2nM). Carbachol caused contraction (EC50=0.3µM), responses were antagonised more potently by 4-DAMP (M3-antagonist) than methoctramine (M2-antagonist). Electrical field stimulation caused contraction, which was inhibited by atropine (60%) and less by guanethidine and α,β-methylene-ATP. The neurogenic responses were not potentiated by inhibition of nitric oxide synthase. Presence of an intact urothelium significantly depressed responses to carbachol (p=0.02) and addition of indomethacin and L-NNA to remove prostaglandin and nitric oxide production respectively did not prevent the inhibitory effect of the urothelium. In conclusion, b-receptor agonists cause relaxation and muscarinic agonists cause contraction via the M3-receptor. Acetylcholine is the main neurotransmitter causing contraction while nitric oxide has a minor role. The mouse and human urothelium are similar in releasing a factor that inhibits contraction of the detrusor muscle which is unidentified but is not nitric oxide or a prostaglandin. Therefore, the mouse may be used as a model to study the lower urinary tract.

Keywords

EN

Mouse; Urinary bladder; Urothelium; Contraction; Relaxation

• Publisher: De Gruyter Open
• Journal: Open Medicine
• Year: 2009
• Volume: 4
• Issue: 2
• Pages: 192-197

Dates

published

1 - 6 - 2009

online

27 - 3 - 2009

Contributors

author

A. Canda

• Department of Biomedical Science, University of Sheffield, S102JF, Sheffield, UK

author

Christopher Chapple

• Department of Urology, The Royal Hallamshire Hospital, S102JF, Sheffield, UK

author

Russ Chess-Williams

• Department of Biomedical Science, University of Sheffield, S102JF, Sheffield, UK

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Identifiers

DOI

10.2478/s11536-008-0082-2