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Number of results

Journal

2009 | 4 | 2 | 192-197

Article title

Pharmacologic responses of the mouse urinary bladder

Content

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Languages of publication

EN

Abstracts

EN
The aim of the study was to determine pathways involved in contraction and relaxation of the mouse urinary bladder. Mouse bladder strips were set up in gassed Krebs-bicarbonate solution and responses to various drugs and electrical field stimulation were obtained. Isoprenaline (b-receptor agonist) caused a 63% inhibition of carbachol precontracted detrusor (EC50=2nM). Carbachol caused contraction (EC50=0.3µM), responses were antagonised more potently by 4-DAMP (M3-antagonist) than methoctramine (M2-antagonist). Electrical field stimulation caused contraction, which was inhibited by atropine (60%) and less by guanethidine and α,β-methylene-ATP. The neurogenic responses were not potentiated by inhibition of nitric oxide synthase. Presence of an intact urothelium significantly depressed responses to carbachol (p=0.02) and addition of indomethacin and L-NNA to remove prostaglandin and nitric oxide production respectively did not prevent the inhibitory effect of the urothelium. In conclusion, b-receptor agonists cause relaxation and muscarinic agonists cause contraction via the M3-receptor. Acetylcholine is the main neurotransmitter causing contraction while nitric oxide has a minor role. The mouse and human urothelium are similar in releasing a factor that inhibits contraction of the detrusor muscle which is unidentified but is not nitric oxide or a prostaglandin. Therefore, the mouse may be used as a model to study the lower urinary tract.

Publisher

Journal

Year

Volume

4

Issue

2

Pages

192-197

Physical description

Dates

published
1 - 6 - 2009
online
27 - 3 - 2009

Contributors

author
  • Department of Biomedical Science, University of Sheffield, S102JF, Sheffield, UK
  • Department of Urology, The Royal Hallamshire Hospital, S102JF, Sheffield, UK
  • Department of Biomedical Science, University of Sheffield, S102JF, Sheffield, UK

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_s11536-008-0082-2
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