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Number of results

Journal

2010 | 8 | 1 | 19-27

Article title

Design of an amperometric xanthine biosensor based on a graphite transducer patterned with noble metal microparticles

Content

Title variants

Languages of publication

EN

Abstracts

EN
A mesoporous graphite material micro-structured with palladium-platinum deposits (mixed in the ratio of 70:30% Pd:Pt) has been used as a cost-effective electrode material for designing an amperometric biosensor for xanthine. The here reported biosensor shows significantly improved operational parameters as compared to previously published results. At a constant applied potential of −0.05 V (vs. Ag/AgCl) it is distinguished with enhanced selectivity of the determination: at the working potential the current from the electrochemical transformation of various electrochemically active substances usually attending biological fluids (incl. uric acid, L-ascorbic acid, glutathione and paracetamol) has been eliminated. The effect of both the temperature and buffer composition on the analytical performance of the sensor has been investigated. Under optimal operational conditions (25°C, −0.05 V vs. Ag/AgCl, phosphate buffer, pH 8.4), the following have been defined for the biosensor: sensitivity 0.39 µA µM−1, strict linearity of the response up to xanthine concentration 70 µM, detection limit of 1.5 µM (S/N=3) and a response time of at most 60 s. [...]

Publisher

Journal

Year

Volume

8

Issue

1

Pages

19-27

Physical description

Dates

published
1 - 2 - 2010
online
16 - 2 - 2010

Contributors

  • Department of Inorganic and Physical Chemistry, University of Food Technology, 4002, Plovdiv, Bulgaria
  • Department of Physical Chemistry, Plovdiv University, 4000, Plovdiv, Bulgaria
author
  • Department of Physical Chemistry, Plovdiv University, 4000, Plovdiv, Bulgaria

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_s11532-009-0102-3
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