Design of an amperometric xanthine biosensor based on a graphite transducer patterned with noble metal microparticles

• Authors: Totka Dodevska , Elena Horozova , Nina Dimcheva
• Languages of publication: EN

Abstracts

EN

A mesoporous graphite material micro-structured with palladium-platinum deposits (mixed in the ratio of 70:30% Pd:Pt) has been used as a cost-effective electrode material for designing an amperometric biosensor for xanthine. The here reported biosensor shows significantly improved operational parameters as compared to previously published results. At a constant applied potential of −0.05 V (vs. Ag/AgCl) it is distinguished with enhanced selectivity of the determination: at the working potential the current from the electrochemical transformation of various electrochemically active substances usually attending biological fluids (incl. uric acid, L-ascorbic acid, glutathione and paracetamol) has been eliminated. The effect of both the temperature and buffer composition on the analytical performance of the sensor has been investigated. Under optimal operational conditions (25°C, −0.05 V vs. Ag/AgCl, phosphate buffer, pH 8.4), the following have been defined for the biosensor: sensitivity 0.39 µA µM−1, strict linearity of the response up to xanthine concentration 70 µM, detection limit of 1.5 µM (S/N=3) and a response time of at most 60 s. [...]

Keywords

EN

Xanthine; Biosensor; Hydrogen peroxide; Electroreduction; Modified graphite electrodes

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2010
• Volume: 8
• Issue: 1
• Pages: 19-27

Dates

published

1 - 2 - 2010

online

16 - 2 - 2010

Contributors

author

Totka Dodevska

• Department of Inorganic and Physical Chemistry, University of Food Technology, 4002, Plovdiv, Bulgaria

author

Elena Horozova

• Department of Physical Chemistry, Plovdiv University, 4000, Plovdiv, Bulgaria

author

Nina Dimcheva

• Department of Physical Chemistry, Plovdiv University, 4000, Plovdiv, Bulgaria

References

• [1] H. Shahbazian, H. Mombini, A. Moghaddam, M. Jasemi, Urology Journal 3, 225 (2006)
• [2] S. Sugase, T. Tsuda, Bunseki Kagaku 51, 429 (2002) http://dx.doi.org/10.2116/bunsekikagaku.51.429[Crossref]
• [3] V.F. Samanidou, A.S. Metaxa, I.N. Papadoyannis, Journal of Liquid Chromatography & Related Technologies 25, 43 (2002) http://dx.doi.org/10.1081/JLC-100108538[Crossref]
• [4] L. Monser, Chromatographia 59, 455 (2004) http://dx.doi.org/10.1365/s10337-004-0223-y[Crossref]
• [5] M.C. Di Pietro, D. Vannoni, R. Leoncini, G. Liso, R. Guerranti, E. Marinello, J. Chrom. B: Biomed. Sci. Appl. 751, 87 (2001) http://dx.doi.org/10.1016/S0378-4347(00)00471-0[Crossref]
• [6] J. Hlavay, S. Haemmerli, G. Guilbault, Biosensors and Bioelectronics 9, 189 (1994) http://dx.doi.org/10.1016/0956-5663(94)80121-5[Crossref]
• [7] Z.K. Shihabi, M.E. Hinsdale, A.J. Bleyer, J. Chrom. B: Biomed. Sci. Appl. 669, 163 (1995) http://dx.doi.org/10.1016/0378-4347(95)00063-O[Crossref]
• [8] P. Britz-McKibbin, D.Y. Chen, Chromatographia 57, 87 (2003) http://dx.doi.org/10.1007/BF02497482[Crossref]
• [9] J.M. Amigo, J. Coello, S. Maspoch, Anal. Bioanal. Chem. 382, 1380 (2005) http://dx.doi.org/10.1007/s00216-005-3275-4[Crossref]
• [10] J. Zhao, J.P. O’Daly, R.W. Henkens, J. Stonehuerner, A.L. Crumbliss, Biosensors and Bioelectronics 11, 493 (1996) http://dx.doi.org/10.1016/0956-5663(96)86786-8[Crossref]
• [11] J.H. Pei, X.Y. Li, Analytica Chimica Acta 414, 205(2000) http://dx.doi.org/10.1016/S0003-2670(00)00775-3[Crossref]
• [12] O. Doblhoff-Dier, G.A. Rechnitz, Analytical Letters 22, 1047 (1989) [Crossref]
• [13] T. Hoshi, T. Noguchi, J. Anzai, Mater. Sci. Eng. C 26, 100 (2006) http://dx.doi.org/10.1016/j.msec.2005.06.001[Crossref]
• [14] Y. Liu, L. Nie, W. Tao, S. Yao, Electroanalysis 16, 1271 (2004) http://dx.doi.org/10.1002/elan.200302947[Crossref]
• [15] E. Kilinc, A. Erdem, L. Gokgunnec, T. Dalbasti, M. Karaoglan, M. Ozsoz, Electroanalysis 10, 273 (1998) http://dx.doi.org/10.1002/(SICI)1521-4109(199804)10:4<273::AID-ELAN273>3.0.CO;2-O[Crossref]
• [16] F. Arslan, A. Yasar, E. Kilic, Artificial Cell Blood Substances 34, 113 (2006) http://dx.doi.org/10.1080/10731190500430289[Crossref]
• [17] Y. Miao, X. Wu, J. Chen, Chemia Analityczna (Warsaw) 50, 507 (2005)
• [18] A.M. Rahman, M.S. Won, Y.B. Shim, Electroanalysis 19, 631 (2007) http://dx.doi.org/10.1002/elan.200603799[Crossref]
• [19] M.S. Ibrahim, Y.M. Temerk, M.M. Kamal, G.A.W. Ahmed, H.S.M. Ibrahim, Microchimica Acta 144, 249 (2004) http://dx.doi.org/10.1007/s00604-003-0118-x[Crossref]
• [20] F. Xiao, C. Ruan, J. Li, L. Liu, F. Zhao, B. Zeng, Electroanalysis 20, 361 (2007) http://dx.doi.org/10.1002/elan.200704042[Crossref]
• [21] Y. Sun, J. Fei, K. Wu, S. Hu, Anal. Bioanal. Chem. 375, 544 (2003)
• [22] N. Dimcheva, E. Horozova, Z. Jordanova, Zeitschrift fur Naturforschung 57c, 883 (2002)
• [23] T. Dodevska, E. Horozova, N. Dimcheva, Anal. Bioanal. Chem. 386, 1413 (2006) http://dx.doi.org/10.1007/s00216-006-0682-0[Crossref]
• [24] E. Horozova, T. Dodevska, N. Dimcheva, Journal of the University of Chemical Technology and Metallurgy 43, 59 (2008) (open access article http://www.uctm.edu/journal/j2008-1/8_Horozova_Dodevska-59-64.pdf)
• [25] D.L. Purich, Methods in Enzymology 354, 1 (2002) http://dx.doi.org/10.1016/S0076-6879(02)54003-9[Crossref]

Identifiers

DOI

10.2478/s11532-009-0102-3