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Number of results
2013 | 1 | 39-55
Article title

Bis-pyridinium quadrupolar derivatives.
High Stokes shift selective probes for bio-imaging

Content
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Languages of publication
EN
Abstracts
EN
We describe the design, synthesis and characterization of
five high Stokes shift quadrupolar heteroaryl compounds suitable
as fluorescent probes in bio-imaging. In particular, we
characterize the photophysical properties and the intracellular
localization in Human Umbilical Vein Endothelial Cells
(HUVEC) and Human Mesenchymal Stem Cells (HMSCs) for
each dye. We show that, amongst all of the investigated
derivatives, the 2,5-bis[1-(4-N-methylpyridinium)ethen-2-yl)]-
N-methylpyrrole salt is the best candidates as selective mitochondrial
tracker. Finally, we recorded the full emission spectrum
of the most performing - exclusively mitochondrial selective
- fluorescent probe directly from HUVEC stained cells.
The emission spectrum collected from the stained mitochondria
shows a remarkably more pronounced vibronic structure
with respect to the emission of the free fluorophore in solution.
Publisher

Year
Volume
1
Pages
39-55
Physical description
Dates
online
05 - 11 - 2013
received
15 - 6 - 2013
accepted
17 - 7 - 2013
Contributors
  • Department of Materials Science and INSTM,
    University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy
  • CNR-ISTM, Institute of Molecular Science and Technology,
    via Golgi 19, I-20133 Milan, Italy
  • CNR-ISTM, Institute of Molecular Science and Technology,
    via Golgi 19, I-20133 Milan, Italy
  • Department of Materials Science and INSTM,
    University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy
  • Department of Materials Science and INSTM,
    University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy
author
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_oph-2013-0005
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